Hesperetin

Manufactured by Cayman Chemical

Sourced in United States

Hesperetin is a flavonoid compound derived from citrus fruits. It functions as a reference standard for analytical chemistry and biochemical research applications.

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Lab products found in correlation

3 protocols using hesperetin

Polyphenol Standards and Isotope-Labeled Compounds

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Myricetin, 3,4-dihydroxy phenyl ethanol, homovanillic acid sulfate, equol, genistein, phloretin, kaempferol, enterolactone, hesperetin, isorhamnetin, protocatechuic acid, trans-resveratrol, roseoflavin, pyrogallol, isoferulic acid, urolithin A, phloroglucinol, (R)-γ-valerolactone and freight were purchased from Cayman company (Ann Arbor, MI, USA). Other polyphenol standards were purified in-house through chromatographic methods with mass spectrometry/NMR confirmation. Authentic standards corresponding to the measured metabolites were purchased from Sigma-Aldrich (Saint Louis, MO, USA) or IROA Technologies (Boston, MA, USA). The stable isotope-labeled amino acid mix (20 AA U-13C, 97–99%; U-15N, 97–99%) was purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA). HPLC-MS-grade acetonitrile, ammonium acetate, and acetic acid were all purchased from Fisher Scientific (Pittsburgh, PA, USA).

Xu M., Yang K, & Zhu J. (2020). Monitoring the Diversity and Metabolic Shift of Gut Microbes during Green Tea Feeding in an In Vitro Human Colonic Model. Molecules, 25(21), 5101.

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Hesperetin-Cyclodextrin Complexation for Anti-Inflammatory and Anti-Cancer Applications

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Hesperetin was purchased from Cayman Chemicals (Ann Arbor, MI, USA) and β-CD and RAMEB were purchased from Wako Pure Chemical Industries (Osaka, Japan), respectively. Naringenin, DM-β-CD, dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), thiazolyl blue (MTT), compounds for phosphate buffer saline (PBS) and lipopolysaccharides (LPS) from Escherichia coli O111:B4 were obtained from Sigma-Aldrich (Darmstadt, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) was obtained from Life Technologies (Carlsbad, CA, USA). Anti-Mouse IL-6 was purchased from eBioscience Inc. (San Diego, CA, USA). Human colon cancer (Caco-2) cells, breast cancer (MCF-7) cells and human cervical carcinoma (HeLa) cells were obtained from the American Type Cell Culture Collection (ATCC), USA.

Sangpheak W., Kicuntod J., Schuster R., Rungrotmongkol T., Wolschann P., Kungwan N., Viernstein H., Mueller M, & Pongsawasdi P. (2015). Physical properties and biological activities of hesperetin and naringenin in complex with methylated β-cyclodextrin. Beilstein Journal of Organic Chemistry, 11, 2763-2773.

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Hesperetin Inhibits TGF-β1-Induced Cytoskeletal Remodeling

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Hesperetin (purity ≥ 98%), purchased from Cayman Chemical Company (Michigan, USA), was dissolved in dimethyl sulfoxide (DMSO) and stored at −20°C. Transforming growth factor-β1 (TGF-β1) purchased from Wako (Tokyo, Japan) was made up at 40 μg/mL and stored at −80°C before use. The final concentration of 20 ng/mL of TGF-β1 was used in this study. Rhodamine phalloidin and DAPI (NucBlue^® Fixed Cell Stain ReadyProbe™ reagent) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The primary and second antibodies used were as follows: Fyn (1:1000, BD Biosciences, USA), p-Src (Y416) (1:1000, Cell Signaling Technology, USA), paxillin (1:5000, BD Biosciences, USA), p-paxillin (Y31) (1:500, Santa Cruz, USA), p-paxillin (Y88) (1:500, Santa Cruz, USA), p-paxillin (Y118) (1:500, Santa Cruz, USA), RhoA (1:500, Santa Cruz, USA), MYPT-1(H-130) (1:500, Santa Cruz, USA), p-MYPT-1(Thr850) (1:500, Merck Millipore, USA). Anti-mouse IgG HRP Conjugate, anti-rabbit IgG HRP Conjugate, and anti-goat IgG HRP Conjugate (diluted 1:5000 with TBS-T) were purchased from Promega (Madison, WI, USA).

Lu Q., Lai Y., Zhang H., Ren K., Liu W., An Y., Yao J, & Fan H. (2022). Hesperetin Inhibits TGF-β1-Induced Migration and Invasion of Triple Negative Breast Cancer MDA-MB-231 Cells via Suppressing Fyn/Paxillin/RhoA Pathway. Integrative Cancer Therapies, 21, 15347354221086900.

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