Recombinant human scf rhscf

All reagents used for cell culture were purchased from Invitrogen (Gaithersburg, MD, USA). Recombinant mouse interleukin-3 (IL-3), mouse stem cell factor (SCF), and recombinant human SCF (rhSCF) were obtained from Peprotech (Rocky Hill, NJ, USA). Compound 48/80 (C48/80) was obtained from AnaSpec (Fremont, CA, USA). Murepavadin (Catalog HY-P1674A) was from MedChem Express. P-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Pertussis toxin (PTx) was from List Biological Laboratories (Campbell, CA, USA). Fura-2 acetoxymethyl ester was from Abcam (Cambridge, MA, USA). Bright-Glo Luciferase was from Promega (Madison, WI, USA). Phycoerythrin (PE)-conjugated anti-human MRGPRX2 antibody was from BioLegend (San Diego, CA, USA). Amaxa Nucleofector Kit V was from Lonza (Gaithersburg, MD, USA). DuoSet ELISA kits were from R&D Systems (Minneapolis, MN, USA). Hemagglutinin (HA)-tagged MRGPRX2 plasmid in pReceiver-MO6 vector was obtained from GeneCopoeia (Rockville, MD, USA). MRGPRX2-Tango plasmid (Addgene no. 66440) was a gift from Dr. Bryan Roth.

All cell culture reagents were purchased from Invitrogen (Carlsbad, CA, USA). Amaxa Nucleofector kit (Kit V) was obtained from Lonza (Gaithersburg, MD, USA). Recombinant mouse interleukin-3 (IL-3) and stem cell factor (SCF) and recombinant human SCF (rhSCF) were purchased from Peprotech (Rocky Hill, NJ, USA). Rocuronium bromide (CAS no. 119302-91-9, Item no. 23698) was purchased from Cayman Chemical (Ann Arbor, MI, USA). p-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) was from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated anti-human LAMP-1 and phycoerythrin (PE)-conjugated anti-human MRGPRX2 antibodies were from BioLegend (San Diego, CA, USA). Plasmid encoding hemagglutinin (HA)-tagged human MRGPRX2 in pReceiver-MO6 vector was obtained from GeneCopoeia (Rockville, MD, USA).