Cytospin slides were prepared (50 000 cells/slide) and were stained with MGG as described.33 (link) TEM analysis was performed as described previously using four million cells per sample.36 (link) For tryptase staining, cytospin slides were incubated for 30 min with a solution of 10 mM Z-Gly-Pro-Arg 4-methoxy-2-naphtylamine (Sigma-Aldrich) in 0.5 M Tris-HCl (pH 7.5) and 5 mg/ml Fast Garnet GBC sulfate salt (Sigma-Aldrich). Cytospin slides were mounted Slowfade Gold antifade reagent (ThermoFisher Scientific) and inspected by light microscopy.
Z gly pro arg 4 methoxy 2 naphtylamine
Manufactured by Merck Group
Z-Gly-Pro-Arg 4-methoxy-2-naphtylamine is a synthetic compound used in various laboratory applications. It serves as a substrate for the measurement of enzymatic activity, particularly related to proteases. The compound provides a quantifiable signal upon enzymatic cleavage, enabling researchers to study and analyze the kinetics and characteristics of these enzymes.
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Lab products found in correlation
2 protocols using z gly pro arg 4 methoxy 2 naphtylamine
1
Cytospin, TEM, and Tryptase Staining
2
Tryptase Activity and Immunohistochemical Staining
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