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Anti granzyme b pe clone gb11

Manufactured by BD
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Anti-granzyme B-PE (clone GB11) is a lab equipment product that detects the presence of granzyme B in cells. Granzyme B is a serine protease involved in cell-mediated immune responses. This product uses the GB11 clone of the anti-granzyme B antibody conjugated to the fluorescent dye phycoerythrin (PE) to enable detection of granzyme B-positive cells.

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Lab products found in correlation

Anti cd3 cd28 beads, by Thermo Fisher Scientific (1 mentions) Cflow plus, by BD (1 mentions) Anti ifn γ apc clone b27, by BD (1 mentions) Accuri c6, by BD (1 mentions)

Spelling variants of this product

Granzyme B (39 citations) Anti-granzyme B (17 citations) Granzyme B (GB11) (7 citations) Granzyme B-PE (7 citations) Anti-Granzyme B-PE (5 citations) Granzyme B-PE (GB11; (3 citations) Anti-granzyme B (GB11; (3 citations) PE-CF594-anti-granzyme B (clone GB11, (3 citations) Anti-granzyme B (clone GB11, (2 citations) Granzyme B (clone GB11, (2 citations)

2 protocols using anti granzyme b pe clone gb11

1

Cytotoxic Lymphocyte Activation Kinetics

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CTL-JB4 were stimulated in M or L with anti-CD3/CD28 beads (Dynal) at a cell to bead ratio of 1:1 for 4 h. Cell suspensions were harvested at 0, 1, 2, 3, and 4 h and stained after fixation (1% paraformaldehyde) and permeabilization (0.1% and 0.3% saponin) with anti-perforin-FITC (clone dG8, BD Pharmingen), anti-granzyme B-PE (clone GB11, BD Pharmingen) and anti-granzyme A-FITC (clone CB9, BD Bioscience). Data acquisition and analysis employed the LSRII (BD Pharmingen) and FlowJo 7.6.5. The MFI were recorded and set in relation to the 0 h time point of M or L, respectively.
Fischbeck A.J., Ruehland S., Ettinger A., Paetzold K., Masouris I., Noessner E, & Mendler A.N. (2020). Tumor Lactic Acidosis: Protecting Tumor by Inhibiting Cytotoxic Activity Through Motility Arrest and Bioenergetic Silencing. Frontiers in Oncology, 10, 589434.
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2

Flow Cytometry Analysis of Lymphocyte Markers

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All flow cytometry experiments were run on an Accuri C6 (BD Biosciences, Inc.) flow cytometer. Antibodies used were as follows: anti-BR3 PE, clone 11C1, BD Biosciences; anti-TACI APC, clone 165004, and anti-BCMA APC, goat pAb FAB193A, both from RnD Systems; anti-CD25 FITC-Violet and APC, clone 3H3, Miltenyi Biotec; anti-CD69 FITC, clone FN50, eBioscience; anti-IFN-γ APC, clone B27, BD Biosciences; anti-Granzyme B PE, clone GB11, BD Biosciences; anti-CRTAM PE, clone Cr24.1, Biolegend, Inc; anti-BAFF APC, clone 1D6, BD Biosciences; anti-BCMA APC, pAb FAB193A, RnD Systems, Inc; anti-cleaved PARP (Asp214) PE, clone XF21-852, BD Biosciences; anti-active Caspase 3 FITC, clone C92-605, BD Biosciences. For intracellular staining of IFN-γ, cells stimulated with plate-bound anti-CD3/CD28 for 12 hours were cultured in the presence of Brefeldin A for another 4-6 hours. Cells were then fixed and permeabilized using reagents from RnD Systems (FC004 Fixation Buffer, FC005 Permeabilization Buffer) according to manufacturer’s instructions. Analyses were performed using CFlowPlus (BD Biosciences) and/or FlowJo (TreeStar, Inc.) software.
Bloom D.D., Reshetylo S., Nytes C., Goodsett C.T, & Hematti P. (2018). Blockade of BAFF Receptor BR3 on T cells Enhances Their Activation and Cytotoxicity. Journal of immunotherapy (Hagerstown, Md. : 1997), 41(5), 213-223.
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