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3 protocols using ampicillin sodium salt

1

CT26 Cell Culture and Akkermansia Muciniphila Maintenance

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CT26 cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Roswell Park Memorial Institute (RPMI) 1640 medium and fetal bovine serum (FBS) were purchased from Gibco (Shanghai, China). Ampicillin sodium salt, neomycin sulfate, metronidazole, and vancomycin HCl were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Akkermansia muciniphila was obtained from the BeNa Culture Collection (Henan Province, China). The thioglycollate medium was purchased from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, China).
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2

Broussonetia papyrifera Leaf Extraction and Evaluation

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Fresh Broussonetia papyrifera leaves were collected at the planting base (Beihai, China). Monosaccharide standard was purchased from BoRui Saccharide Biotech Co., Ltd. (Yangzhou, Jiangsu, China). Acetaminophen (AR, 99.0%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Bifendate Pills were purchased from Beijing Xiehe pharmaceutical factory (Beijing, China). Neomycin sulfate, Ampicillin sodium salt, Metronidazole, and Vancomycin hydrochloride were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). AST and ALT test kits were purchased from URIT Medical Electronics Co., Ltd. (Guilin, China). H&E and Hoechst33258 dye solution set were obtained from Servicebio technology Co., Ltd. (Wuhan, China). SOD, MDA, GSH, and GSH-PX test kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Mice IL-6 (MM-0163M1), IL-10 (MM-0176M1), TNF-α (MM-0132M1), CYP2E1 (MM-44419M1), SULT1A1 (MM-0874M1), UGT (MM-0770M1), caspase-3 (MM-43676M1), caspase-8 (MM-45746M1), and caspase-9 (MM-1179M1) ELISA kits were purchased from Jiangsu Meimian industrial Co., Ltd. (Yancheng, Jiangsu, China). Spin Column Animal Total RNA Purification Kit was purchased from Sangon BiotechCo., Ltd. (Shanghai, China), First-strand cDNA Synthesis Mix and RealStar Green Fast Mixture were purchased from GenStar Co., Ltd. (Beijing, China).
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3

Preparation of Gold Nanoparticles

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Hydrogen tetrachloroauric acid (HAuCl 4 ⋅3H 2 O, 99.9%), hexadecyltrimethylammonium bromide (CTAB, 99%), sodium borohydride (NaBH 4 ), L-ascorbic acid (AA), and silver nitrate salt (AgNO 3 , 99.97%), Luria-Bertani (LB), absolute ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Hydrochloric acid (HCl) was purchased from Zhongshi Chemical Co., Ltd (Shanghai, China). Hexadecyltrimethylammonium chloride (CTAC,97%), rhodamine 6G (R6G), DL-thioctic acid (LA), N-hydroxysuccinimide (NHS), glutaraldehyde (50%) were all purchased from Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). Streptavidin were purchased from Solarbio technology Co., Ltd. (Beijing, China). Biotinylated antibody was purchased from Bioss Biotechnology co., Ltd (Beijing, China). N 2 was purchased from Yantai Rulin Gas Co., Ltd (Yantai, China). Ampicillin sodium salt was purchased from Shanghai Macklin Biochemical Co.,Ltd (Shanghai, China). E.coli DH5α was purchased from Takara Biomedical Technology Co., Ltd (Beijing, China). The reagents mentioned above were used as received without any treatment and performed with Milli-Q water.
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