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Engen lbcas12a

Manufactured by New England Biolabs
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Engen LbCas12a is a Cas12a enzyme from Lachnospiraceae bacterium that exhibits robust DNA cleavage activity. It is a member of the Class 2, Type V CRISPR-Cas system.

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Lab products found in correlation

G 25 sephadex column, by Roche (1 mentions) Fluorescein 12 dutp, by Jena Biosciences (1 mentions) Biotin 16 dutp, by Jena Biosciences (1 mentions) Sodium l ascorbate, by Merck Group (1 mentions) Copper 2 sulphate pentahydrate, by Merck Group (1 mentions)

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LbCas12a (25 citations)

2 protocols using engen lbcas12a

1

Enzyme-Mediated DNA Labeling Protocols

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Engen LbCas12a (#M0653T) and TdT (#M0315L) were obtained from New England Biolabs (Ipswich, MA, USA) as were the matching CoCl2 (2.5 mM stock) plus TdT buffer (final concentration: 50 mM potassium acetate, 20 mM Tris–acetate, and 10 mM magnesium acetate; pH 7.9). dTTP (ThermoFisher #R0171, Bleiswijk, The Netherlands). Fluorescein-12-dUTP (Jena Bioscience, Germany) (#NU-803-FAMX-L). Biotin-16-dUTP (Jena Bioscience, Germany) (#NU-803-BIO16-S). HybriDetect, Universal LFA Kit (Milenia Biotec, Gießen Germany). G-25 Sephadex Columns (Roche, #11273949001). Dideoxycytidine (GE Healthcare, USA) (#27-2061-01).
Berghuis N.F., Mars-Groenendijk R., Busker R.W., Paauw A, & van Leeuwen H.C. (2022). Combining CRISPR–Cas12a with terminal deoxynucleotidyl transferase dependent reporter elongation for pathogen detection using lateral flow test strips. Biology Methods & Protocols, 7(1), bpac015.
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2

Enzymatic DNA Modification Assay

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Sodium L-ascorbate (#A7631) and copper(II) sulphate pentahydrate (Sigma-Aldrich, #203165) from Sigma-Aldrich (Zwijndrecht, The Netherlands). Engen LbCas12a (#M0653T) and TdT (#M0315L) were obtained from New England Biolabs (Ipswich, USA) as were the matching CoCl2 (2.5 mM stock) plus TdT buffer (final concentration: 50 mM potassium acetate, 20 mM Tris–acetate, 10 mM magnesium acetate; pH 7.9). dTTP (ThermoFisher #R0171, Bleiswijk, The Netherlands).
Bogers J.F., Berghuis N.F., Busker R.W., van Booma A., Paauw A, & van Leeuwen H.C. (2020). Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles. Biology Methods & Protocols, 6(1), bpaa020.
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