Anti-human fluorochrome-conjugated mAbs known to cross-react with rhesus macaque antigens were used in this study, including PE anti-IL-21 (3A3-N2.1), PE-CF594 anti-CD95 (DX2), PE-Cy5 anti-CD154 (TRAP1), APC anti-CD152 (BNI3) and Alexa Fluor 700 anti-CD3 (SP34-2) (all from BD Biosciences, San Jose, CA); PerCP-eFluor710 anti-CD279 (eBioJ105) and APC-eFluor780 anti-CD197 (3D12) (all from eBioscience, San Diego, CA); PE-Cy7 anti-IL-17A (BL168) and Pacific Blue anti-CD278 (C398.4A) (BioLegend, San Diego, CA); and QDot605 anti-CD4 (T4/19Thy5D7) (NIH Nonhuman Primate Reagent Resource, Boston, MA). The Yellow LIVE/DEAD viability dye (Invitrogen, Carlsbad, CA) was used to exclude dead cells. At least 500,000 singlet events were acquired on a SORP LSRII (BD Biosciences and analyzed using FlowJo Software (FlowJo LLC, Ashland, OR). For all samples gating was established using a combination of isotype and fluorescence-minus-one controls.
Alexa fluor 700 anti cd3 sp34 2
Manufactured by BD
Sourced in United States
Alexa Fluor 700 anti-CD3 (SP34-2) is a fluorescence-labeled antibody that binds to the CD3 antigen. CD3 is a cell surface receptor complex found on T cells. The Alexa Fluor 700 dye is used to label the antibody, allowing for detection and quantification of CD3-expressing cells in flow cytometry applications.
Automatically generated - may contain errors
2 protocols using alexa fluor 700 anti cd3 sp34 2
1
Multiparametric Flow Cytometry of Rhesus Macaque
2
Multicolor Flow Cytometry Analysis
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Anti-human fluorochrome-conjugated monoclonal antibodies known to cross-react with rhesus macaque antigens were used, including FITC anti-CD20 (2H7), APC-Cy7 anti-CD16 (3G8), V450 anti-IFN-γ (B27), Alexa Fluor 700 anti-CD3 (SP34-2), and PerCP-Cy5.5 anti-CD8 (SK1), all from BD Biosciences; eFluor 660 anti-CD107a (eBioH4A3) from eBioscience; PE anti-NKG2A (Z199) from Beckman Coulter (Fullerton, CA, USA); QDot605 anti-CD8 (3B5), and the Yellow and Aqua Live/Dead viability dyes from Invitrogen; and QDot605 anti-CD4 (19Thy-5D7) from the NIH Non-human Primate Reagent Resource (Boston, MA, USA). Cells were stained for specific surface molecules, fixed and permeabilized with a Cytofix/Cytoperm Kit (BD Biosciences), and then stained for specific intracellular molecules. At least 250,000 singlet events (PBMCs) were acquired on a LSR II (BD Biosciences) and analyzed using FlowJo Software (Treestar Inc., Ashland, OR, USA). For all samples, gating was established using a combination of isotype and fluorescence-minus-one controls.
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