To generate NP-specific PCs of different ages, B1-8hi B cells were isolated from spleen and lymph nodes by magnetic cell separation (MACS) using anti-CD43 beads (Miltenyi Biotec), and transferred intravenously to C57BL/6 recipient mice with 3-7x106 B1-8hi B cells or 0.5-1x106 Igλ+ B1-8hi B cells per mouse. One day after the transfer, recipient mice were immunized intraperitoneally with 50 μg of NP(25)-KLH (Biosearch Technologies) emulsified in alum (Imject Alum; Thermo Fisher Scientific) at 2:1 v:v ratio in 150 μL volume for 2-8 weeks.
Np 25 klh
Manufactured by Biosearch Technologies
NP(25)-KLH is a laboratory reagent used in biochemical research. It consists of 25 molecules of the hapten nitrophenyl (NP) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). This product serves as a component in immunological assays and studies.
Automatically generated - may contain errors
3 protocols using np 25 klh
1
Generating NP-specific Plasma Cells
2
Generating NP-specific Plasma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To generate NP-specific PCs of different ages, B1-8hi B cells were isolated from spleen and lymph nodes by magnetic cell separation (MACS) using anti-CD43 beads (Miltenyi Biotec), and transferred intravenously to C57BL/6 recipient mice with 3-7x106 B1-8hi B cells or 0.5-1x106 Igλ+ B1-8hi B cells per mouse. One day after the transfer, recipient mice were immunized intraperitoneally with 50 μg of NP(25)-KLH (Biosearch Technologies) emulsified in alum (Imject Alum; Thermo Fisher Scientific) at 2:1 v:v ratio in 150 μL volume for 2-8 weeks.
3
Immunization of Mice with NP-KLH and Adjuvants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven to eight week old mice were immunized intraperitoneally with 100 μg NP(23)-KLH, NP(25)-KLH or NP(27)-KLH (Biosearch Technologies) mixed with 50% (v/v) imject alum (Thermo Fisher Scientific) and 1 μg LPS (Sigma) as described previously1 ,2 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!