Anti eif2

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-EIF2 is a laboratory reagent that specifically binds to and detects the EIF2 protein. EIF2 is a key component of the eukaryotic translation initiation complex and plays a crucial role in protein synthesis.

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Lab products found in correlation

3 protocols using anti eif2

Comprehensive Protein Expression Analysis

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Anti-CHOP, anti-AKT, anti-Caspase3, anti- EIF2, anti-PERK, anti-XBP1, anti-GAPDH, anti-GRP78, anti-Bax, anti-Bak, anti-Bcl2, anti-Bim, anti-PUMA (Cell Signaling Technology, MA, USA). Goat anti-mouse and anti-rabbit antibodies conjugated with horseradish peroxidase were secondary antibodies (1:2,000, Jackson ImmunoResearch, PA, USA).

Zi J., Han Q., Gu S., McGrath M., Kane S., Song C, & Ge Z. (2020). Targeting NAT10 Induces Apoptosis Associated With Enhancing Endoplasmic Reticulum Stress in Acute Myeloid Leukemia Cells. Frontiers in Oncology, 10, 598107.

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Optimization and Validation of LMPTP Inhibitor

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LMPTP inhibitor Compd. 23 was generated as described (14 (link)) and formulated in rodent chow at 0.1% (w/w) by Research Diets. CRISPR-Cas9 plasmids were purchased from ATUM. Pierce d-Luciferin monopotassium salt was purchased from Thermo Fisher Scientific and reconstituted using phosphate-buffered saline (PBS). For in vitro experiments, docetaxel and cabazitaxel were purchased from Selleckchem and reconstituted in sterile dimethyl sulfoxide (DMSO). The anti–phospho–eIF2-Ser⁵¹ (#3398), anti-eIF2 (#9722), anti-NRF2 (#12721), anti-ATF4 (#11815), anti–glyceraldehyde 3-phosphate dehydrogenase (#5174), anti–phospho-H2AX (#9718), anti-H2AX (#2595), and anti-pTyr (pY1000; #8954) antibodies were purchased from Cell Signaling Technology (CST). The rabbit anti-LMPTP antibody was described in (52 (link)). The mouse anti-LMPTP antibody (#sc-100343) was purchased from Santa Cruz Biotechnology. The anti-GSS antibodies were purchased from Thermo Fisher Scientific (#PA5-89891) or Santa Cruz Biotechnology (#sc-166882). Anti-rabbit (#NA934-1ML) and anti-mouse (#NA931-1ML) secondary antibodies were purchased from Thermo Fisher Scientific. TrueBlot anti-rabbit (#RL18-8816-31) and anti-mouse (#RL18-8817-31) secondary antibodies were purchased from Rockland. GSH-MEE (#353905) was purchased from Sigma-Aldrich. Unless otherwise specified, chemicals and other reagents were purchased from Sigma-Aldrich.

Stanford S.M., Nguyen T.P., Chang J., Zhao Z., Hackman G.L., Santelli E., Sanders C.M., Katiki M., Dondossola E., Brauer B.L., Diaz M.A., Zhan Y., Ramsey S.H., Watson P.A., Sankaran B., Paindelli C., Parietti V., Mikos A.G., Lodi A., Bagrodia A., Elliott A., McKay R.R., Murali R., Tiziani S., Kettenbach A.N, & Bottini N. (2024). Targeting prostate tumor low–molecular weight tyrosine phosphatase for oxidation-sensitizing therapy. Science Advances, 10(5), eadg7887.

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Retinal Protein Extraction and Analysis

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Retinas were lysed as previously described to obtain nuclear-enriched proteins and total cellular proteins [24] . The purity of enriched lysates was checked by immunoblotting using a nuclear marker (anti-Histone H3 1:3000; Bethyl Laboratories) or a cytosol marker (antipan-actin, 1:3000; Millipore). Equivalent amounts of protein extracts (20 g) were resolved using SDS-PAGE and immunoblottings were performed following standard procedures. The antibodies used for western blotting were: anti-II-spectrin (AA6, 1:2000; Enzo Life) [25] , anti-AIF (1: 1000; Calbiochem) [26] , anti-Caspase7 (1:1000; Cell Signaling), anti-eIF2 (1:1000; Cell Signaling), anti-NRF2 (1:2000; Invitrogen), anti-phosphorylated-IRE1 (1:2000; Novus Biologicals), anti-phosphorylated-PERK (1:1000; Cell Signaling), antiphosphorylated-eIF2 (1:1000; Cell Signaling). Antibodies specificity is shown in Fig. S2.
Each blot analyzed proteins derived from 4 retinas pooled together and 3 independent pools from 3 different litters were used as biological replicates, one representative blot is shown.
The entire blots are shown in Fig. S3.

Comitato A., Schiroli D., Montanari M, & Marigo V. (2020). Calpain Activation Is the Major Cause of Cell Death in Photoreceptors Expressing a Rhodopsin Misfolding Mutation. Molecular neurobiology, 57(2).

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