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Mbf cx9000 camera

Manufactured by MBF Biosciences
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Sourced in United States, Japan

The MBF CX9000 is a high-resolution camera designed for scientific and microscopy applications. It features a CMOS sensor and is capable of capturing images with a resolution up to 9 megapixels. The camera is compatible with a variety of microscope systems and can be used for various imaging tasks.

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Lab products found in correlation

Dm4000 b microscope, by MBF Biosciences (2 mentions) Dab substrate kit for peroxidase, by Vector Laboratories (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Bx51 microscope, by Olympus (2 mentions) Neurolucida, by MBF Biosciences (1 mentions) Stereo investigator software, by MBF Biosciences (1 mentions)

Close spelling variants of this product

CX9000 camera (5 citations) CX9000 (9 citations)

4 protocols using mbf cx9000 camera

1

Immunohistochemical Staining Protocol

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The immunohistochemical staining procedure started with the incubation in 1% hydrogen peroxidase (in PBS:Methanol 1:1) followed by 3 times of 15 min washing with PBST and 30 min incubation in 7% normal horse serum in 0.02% PBST (blocking solution). Sections were incubated ON with the desired primary antibody in blocking solution at 4 °C. The next day, sections were washed 3 times for 15 min with 0.02% PBST, followed by incubation with the secondary biotinylated antibody for 30 min (Vectastain Elite ABC Kit, Vector laboratories). After three 15 min washing steps with 0.02% PBST and incubation for 30 min in an avidin-biotin complex solution (Vectastain Elite ABC Kit), sections were washed two times for 15 min in 0.02% PBST and twice for 15 min in PBS before stained with 3,3′-diaminobenzidine tetra hydrochloride (DAB) solution (DAB substrate Kit for Peroxidase, Vector laboratories, Burlingame, USA). Staining reactions were terminated upon visual inspection by adding and washing with water. Sections were finally air-dried and mounted with Mowiol. Bright field colored images were captured with an automated Leica DM4000 B microscope coupled to a MBF CX9000 camera (MBF Bioscience) and displayed with PictureFrame™ software.
Vicuña L., Strochlic D.E., Latremoliere A., Bali K.K., Simonetti M., Husainie D., Prokosch S., Riva P., Griffin R.S., Njoo C., Gehrig S., Mall M.A., Arnold B., Devor M., Woolf C.J., Liberles S.D., Costigan M, & Kuner R. (2015). The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. Nature medicine, 21(5), 518-523.
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2

Histological Analysis of Follicle-Sinus-Complexes

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Whole trunks or trunk tips were fixed in 4% formaldehyde for several months. Some of the samples were frozen and thawed prior to fixation. We either dissected Follicle-Sinus-Complexes (FSCs) from the surrounding tissue or cut out small cubes of tissue containing one or multiple FSCs. For cryosectioning, the tissue was transferred into a 30% sucrose solution in phosphate buffer and left for at least 24 h prior to sectioning for cryoprotection. We then embedded the tissue in tissue freezing medium (Leica Biosystems, Catalog Nr. 14020108926) and cut it into 40 µm sections either perpendicular or parallel to the skin surface using a freezing microtome. For hematoxylin-eosin staining sections were mounted and dried for at least 24 h, directly followed by staining with hematoxylin-eosin solution. Pictures of the hematoxylin-eosin stainings were acquired with a MBFCX9000 camera (MBF Bioscience, Williston, USA) on an Olympus BX51 microscope (Olympus, Japan) using Neurolucida (MBF Bioscience, Williston, ND) software. We described characteristic structures and overall anatomy of the FSC using hematoxylin-eosin stained sections from n = 8 follicles (from Hoa’s Baby, Burma, Unknown Asian and Zimba, see Table 1).
Deiringer N., Schneeweiß U., Kaufmann L.V., Eigen L., Speissegger C., Gerhardt B., Holtze S., Fritsch G., Göritz F., Becker R., Ochs A., Hildebrandt T, & Brecht M. (2023). The functional anatomy of elephant trunk whiskers. Communications Biology, 6, 591.
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3

Immunohistochemical Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The immunohistochemical staining procedure started with the incubation in 1% hydrogen peroxidase (in PBS:Methanol 1:1) followed by 3 times of 15 min washing with PBST and 30 min incubation in 7% normal horse serum in 0.02% PBST (blocking solution). Sections were incubated ON with the desired primary antibody in blocking solution at 4 °C. The next day, sections were washed 3 times for 15 min with 0.02% PBST, followed by incubation with the secondary biotinylated antibody for 30 min (Vectastain Elite ABC Kit, Vector laboratories). After three 15 min washing steps with 0.02% PBST and incubation for 30 min in an avidin-biotin complex solution (Vectastain Elite ABC Kit), sections were washed two times for 15 min in 0.02% PBST and twice for 15 min in PBS before stained with 3,3′-diaminobenzidine tetra hydrochloride (DAB) solution (DAB substrate Kit for Peroxidase, Vector laboratories, Burlingame, USA). Staining reactions were terminated upon visual inspection by adding and washing with water. Sections were finally air-dried and mounted with Mowiol. Bright field colored images were captured with an automated Leica DM4000 B microscope coupled to a MBF CX9000 camera (MBF Bioscience) and displayed with PictureFrame™ software.
Vicuña L., Strochlic D.E., Latremoliere A., Bali K.K., Simonetti M., Husainie D., Prokosch S., Riva P., Griffin R.S., Njoo C., Gehrig S., Mall M.A., Arnold B., Devor M., Woolf C.J., Liberles S.D., Costigan M, & Kuner R. (2015). The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. Nature medicine, 21(5), 518-523.
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4

Microscopic Imaging and Analysis Workflow

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Thin Nissl or osmium tetroxide–stained sections were viewed using Stereo Investigator software (MBF Bioscience, Williston, USA) using an Olympus BX51 microscope (Olympus, Japan) with a MBFCX9000 camera (MBF Bioscience, Williston, USA) mounted on the microscope. The microscope was equipped with a motorized stage (LUDL Electronics, Hawthorne, USA) and a z-encoder (Heidenhain, Schaumburg, USA). Stereo Investigator software was used for stereological procedures, cell size, and axon diameter measurement and for acquiring images. Digitized images were adjusted for brightness and contrast using Adobe Photoshop (Adobe Systems Inc., San Jose, CA, USA), but they were not otherwise altered.
Kaufmann L.V., Schneeweiß U., Maier E., Hildebrandt T, & Brecht M. (2022). Elephant facial motor control. Science Advances, 8(43), eabq2789.
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