Szx18

Manufactured by Olympus

The SZX18 is a stereo zoom microscope designed for a wide range of applications. It features a zoom ratio of 18:1 and provides a magnification range of 0.75x to 13.5x. The SZX18 utilizes an Apochromatic optical system to deliver high-resolution, high-contrast images. The microscope is equipped with a coded zoom function that automatically adjusts the illumination settings as the magnification is changed.

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Lab products found in correlation

Ds ri2, by Nikon (1 mentions) Bm purple ap substrate, by Roche (1 mentions)

3 protocols using szx18

Visualizing BnaA3.NIP5;1 Expression in Rapeseed Roots

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Rapeseed root samples from 5-d-old seedlings grown under B-deficiency conditions were fixed with a solution consisting of 63% (v/v) ethanol, 5% (v/v) acetic acid and 2% (v/v) formaldehyde for 4 h, embedded into 5% (w/v) agarose and then sectioned to 50 μm. BnaA3.NIP5;1 in situ RT-PCR flowed method with the modifications of Athman et al. (2014) [46 (link)]. The samples were stained using BM purple AP substrate (Roche) for 30 min, washed in an orderly manner with washing buffer, mounted in 40% (v/v) glycerol and then observed under a microscope (Nikon DS-Ri 2).
The pQ::BnaA3.NIP5;1 fragment was amplified from the pQ::BnaA3.NIP5;1-GFP vector and cloned into pBI121 to generate pQ::BnaA3.NIP5;1-GUS constructs. The resulting vectors were transformed into W10 rapeseed. The pQ::BnaA3.NIP5;1-GUS transgenic seedlings were incubated in a solution of 1 mg ml^-1 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid (X-gluc), 100 mM sodium phosphate (pH 7.0), 0.5 mM K₃Fe(CN)₆, 0.5 mM K₄Fe(CN)₆, 10 mM Na₂EDTA, 0.1% (v/v) Triton X-100 and 20% (v/v) methanol at 37°C in the dark for 1 h. After incubation, the chlorophyll was removed using 75% ethanol, and images were taken using stereomicroscope (Olympus SZX18).

He M., Wang S., Zhang C., Liu L., Zhang J., Qiu S., Wang H., Yang G., Xue S., Shi L, & Xu F. (2021). Genetic variation of BnaA3.NIP5;1 expressing in the lateral root cap contributes to boron deficiency tolerance in Brassica napus. PLoS Genetics, 17(7), e1009661.

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Generation of Fluorescent Arabidopsis Marker Lines

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Regions of approximately À2.5 kb upstream of the transcription starting site of AT1G79710, AT4G18050, AT1G78380, AT4G241 60, AT5G18480, AT4G30390, AT5G54650, AT5G16910, AT5G2 0000, AT2G36580, AT5G56350, and AT5G17760 were amplified from Arabidopsis Col-0 genomic DNA by PCR and cloned into the pGGA (plasmid Green Gate A) entry vector to generate pGGA-pMarkerGene. A region of approximately À1.5 kb upstream of the transcription start site of AT1G30270 was synthetized by GENEWIZ (South Plainfield, NJ) and subsequently also cloned into the pGGA entry vector (Lampropoulos et al., 2013) (link). Each entry vector was then recombined with the following plasmids: pGGB-SV40-NLS, pGGC-3xGFP, pGGD-RBCSt (D-F), pGGF-AlliYFP (seed coat selection cassette for transgenic seed selection), and pGGZ-empty destination vector. Primers used for cloning and sequencing the final constructs are listed in Supplemental Table 12. All plasmids were transfected by electroporation into the Agrobacterium tumefaciens GV3101 strain containing the plasmid pSoup and then transformed into Arabidopsis Col-0 by the floral dipping method (Clough and Bent, 1998) (link). Transgenic seeds from transformed plants were identified as those displaying a clear fluorescence signal under the stereomicroscope (Olympus SZX18).

Salguero-Linares J., Serrano I., Ruiz-Solani N., Salas-Gómez M., Phukan U.J., González V.M., Bernardo-Faura M., Valls M., Rengel D, & Coll N.S. (2022). Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses. Molecular plant, 15(6).

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Construct Generation for Fluorescent Arabidopsis

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A region of approximately -2.5 kb upstream of the transcription starting site of AT5G17760 was amplified from Arabidopsis Col-0 genomic DNA by PCR and cloned into the pGGA (plasmid Green Gate A) entry vector to generate pGGA-pMarkerGene (79) . Each entry vector was then recombined with the following plasmids: pGGB-SV40-NLS, pGGC-3xGFP, pGGD-RBCSt (D-F), pGGF-AlliYFP (seed coat selection cassette for transgenic seed selection) and pGGZ-empty destination vector. Primers used for cloning and sequencing the final constructs are listed in Table S1. All plasmids were transfected by electroporation into Agrobacterium tumefaciens GV3101 strain containing the plasmid pSoup and then transformed into Arabidopsis Col-0 by the floral dipping method (80) . Transgenic seeds from transformed plants were identified as those displaying a clear fluorescence signal under the stereo microscope Olympus SZX18.

Salguero‐Linares J., Serrano I., Ruiz-Solaní N., Salas-Gómez M., Phukan U.J., González V.M., Bernardo-Faura M., Valls M., Rengel D., & Coll N.S. (2021). Robust transcriptional indicators of plant immune cell death revealed by spatio-temporal transcriptome analyses.

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