SH‐SY5Y cells were obtained from the American Type Culture Collection (ATCC CRL‐2266) and cultured in Dulbecco's Modified Eagle Medium (D‐MEM)/Ham's F‐12 with L‐glutamine and phenol red (Cat# 048‐29785; FUJIFILM Wako). They were supplemented with 10% fetal bovine serum (Cat# FB‐1365/500; Biosera) in an incubator with 5% CO2 at 37°C. HEK293 cells were provided by RIKEN BRC through the National BioResource Project of MEXT, Japan. These cells were cultured in D‐MEM (high glucose) with L‐glutamine, phenol red, and sodium pyruvate (Cat# 043‐30085; FUJIFILM Wako), supplemented with 10% fetal bovine serum in an incubator with 5% CO2 at 37°C.
D mem ham s f 12 with l glutamine and phenol red
Manufactured by Fujifilm
Sourced in Japan
D-MEM)/Ham's F-12 with L-glutamine and phenol red is a cell culture medium that supports the growth and maintenance of a variety of cell types. It is a commonly used formulation in research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Sh sy5y cell, by American Type Culture Collection (3 mentions)
Sodium pyruvate, by Fujifilm (2 mentions)
Phenol red, by Fujifilm (2 mentions)
Lenti x 293t cell line hek293t, by Takara Bio (2 mentions)
L glutamine, by Fujifilm (2 mentions)
Fetal bovine serum (fbs), by Fujifilm (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Biosera (1 mentions)
4 protocols using d mem ham s f 12 with l glutamine and phenol red
1
Culturing SH-SY5Y and HEK293 Cells
2
Cultivation of Human Esophageal Cancer Cell Lines
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Human esophageal cancer (SCC) cell lines including T.T, T.Tn, and Kyse-3650 were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). T.T and T.Tn were cultured in a 1:1 mixture of Dulbecco’s modified Eagle’s medium and Ham’s F12 medium (D-MEM/Ham’s F-12 with L-glutamine and phenol red, 048-29785, Wako Pure Chemical Industries, Ltd., Osaka, Japan) containing 10% fetal bovine serum (FBS; 26140079, Gibco®, Thermo Fisher Scientific Inc., Waltham, MA, USA) and 1% antibiotic-antimycotic (15240-062, Gibco®, Thermo Fisher Scientific Inc.). Kyse-3650 was cultured in a 1:1 mixture of Ham’s F12 medium (with L-glutamine and phenol red, 087-08335, Wako Pure Chemical Industries, Ltd.) and Roswell Park Memorial Institute 1640 medium (with L-glutamine and phenol red, 189-02025, Wako Pure Chemical Industries, Ltd.) containing 5% FBS and 1% antibiotic-antimycotic. Cells were cultured in a humidified incubator (MCO-19AIC-UV, Panasonic Corp., Osaka, Japan) at 37°C.
3
Cell Culture Protocols for SH-SY5Y and HEK293
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SH-SY5Y cells were obtained from American Type Culture Collection (ATCC CRL-2266; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (D-MEM)/Ham's F-12 with L-Glutamine and phenol red (Cat# 048-29785; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (Cat# FB-1365/500; Biosera, Nuaille, France) in an incubator with 5% CO2 at 37 °C. HEK293 cells were provided by RIKEN BRC, Tsukuba, Japan (Cat# RCB1637) through the National BioResource Project of MEXT, Japan, and cultured in D-MEM (high glucose) with L-Glutamine, phenol red, and sodium pyruvate (Cat# 043-30085; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum in an incubator with 5% CO2 at 37 °C. The Lenti-X™ 293T cell line (HEK293T) was obtained from Clontech, Mountain View, CA, USA (Cat# 632180) and cultured in the same manner as described above for HEK293 cells.
4
Cell Culture Protocols for SH-SY5Y and HEK293
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SH-SY5Y cells were obtained from American Type Culture Collection (ATCC CRL-2266; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (D-MEM)/Ham's F-12 with L-Glutamine and phenol red (Cat# 048-29785; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (Cat# FB-1365/500; Biosera, Nuaille, France) in an incubator with 5% CO2 at 37 °C. HEK293 cells were provided by RIKEN BRC, Tsukuba, Japan (Cat# RCB1637) through the National BioResource Project of MEXT, Japan, and cultured in D-MEM (high glucose) with L-Glutamine, phenol red, and sodium pyruvate (Cat# 043-30085; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum in an incubator with 5% CO2 at 37 °C. The Lenti-X™ 293T cell line (HEK293T) was obtained from Clontech, Mountain View, CA, USA (Cat# 632180) and cultured in the same manner as described above for HEK293 cells.
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