DNA was extracted using the PowerSoil DNA isolation kit (MoBio, Carlsbad, CA) or a CTAB-based extraction method31 (link). 1 µg of DNA was used to prepare paired-end sequencing libraries using the TruSeq PCR-free kits (Illumina, San Diego, CA, USA) following the manufactures recommendation except that the 550 bp protocol was used with 1 µg of input DNA. Mate-pair libraries were prepared using the Nextera Mate-pair kit (Illumina) using the gel-free approach. The prepared libraries were sequenced using an Illumina MiSeq with MiSeq Reagent Kit v3 (2x301 bp; Illumina).
Miseq with miseq reagent kit v3 2x301 bp
Manufactured by Illumina
The MiSeq with MiSeq Reagent Kit v3 (2x301 bp) is a sequencing system designed for targeted gene sequencing applications. The system enables sequencing of DNA fragments up to 301 base pairs in length from both ends of the fragments, producing paired-end reads.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using miseq with miseq reagent kit v3 2x301 bp
1
Paired-End and Mate-Pair Sequencing
2
Paired-End and Mate-Pair Sequencing
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DNA was extracted using the PowerSoil DNA isolation kit (MoBio, Carlsbad, CA) or a CTAB-based extraction method31 (link). 1 µg of DNA was used to prepare paired-end sequencing libraries using the TruSeq PCR-free kits (Illumina, San Diego, CA, USA) following the manufactures recommendation except that the 550 bp protocol was used with 1 µg of input DNA. Mate-pair libraries were prepared using the Nextera Mate-pair kit (Illumina) using the gel-free approach. The prepared libraries were sequenced using an Illumina MiSeq with MiSeq Reagent Kit v3 (2x301 bp; Illumina).
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