Liquiritin, liquiritigenin, estrone (E1), 17β-estradiol (E2), and estriol (E3) were purchased from FUJIFILM Wako (Japan). Capsaicin, estetrol (E4), and Ani9 were purchased from Sigma-Aldrich (United States). Allyl isothiocyanate, niflumic acid, and dihydrodaidzein were purchased from Tokyo Chemical Industry (Japan).
Allyl isothiocyanate
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Allyl isothiocyanate is a colorless, pungent liquid that is commonly used as a chemical reagent in laboratory settings. It is a member of the isothiocyanate family of compounds. The core function of allyl isothiocyanate is to serve as a versatile and reactive chemical building block in various research and analytical applications.
Automatically generated - may contain errors
3 protocols using allyl isothiocyanate
1
Phytochemical Profiling and Bioactive Compounds
2
Isothiocyanate Compounds and Yeast Cells
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Allyl isothiocyanate (95%), benzyl isothiocyanate (98%), butyl isothiocyanate (98%), ethyl isothiocyanate (97%), isobutyl isothiocyanate (97%), isopropyl isothiocyanate (98%), methyl isothiocyanate (97%), Nile Red, phenyl isothiocyanate (99%), propyl isothiocyanate (98%), and 3-[N-butyl-N-acetyl] aminopropionic acid ethyl ester (IR3535) (95%) were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan) or Sigma-Aldrich Co. (St. Louis, MO, USA). Yeast cells were purchased from Biozoa Biological Supply (Seoul, Korea).
3
Inflammatory Response Imaging in Mice
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A local inflammation model was studied in four mice using a previously described inflammatory reagent, mustard oil (17, 19) . Briefly, dye mixture (equal parts 1% w/v EB and 0.1 mg/mL Genhance 750) was injected into anesthetized mice via the tail vein. At 2 min after dye injection, mustard oil with diluted allyl isothiocyanate (Tokyo Chemical Industry, Co., Ltd., Tokyo, Japan) in mineral oil (MP Biomedicals, LLC., Santa Ana, Calif) was applied to the right footpad (control, left footpad). Fluorescent imaging of right and left footpads was obtained immediately after application (baseline) and 60 min later. After second imaging, mice were euthanized and footpad samples were collected by amputation from the ankles. Collected samples were flash-frozen in liquid nitrogen and stored at -80°C until examination.
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