D 10 hemoglobin a1c program 12000949

Serum samples from the selected participants were thawed prior to biochemical assessments. Serum triglycerides, total cholesterol, high-density lipoprotein (HDL) and cholesterol and glucose levels were analyzed routinely using commercially purchased kits (catalog nos. 981379, 981812, 981823 and 981301, respectively) and an automated biochemical analyzer (Konelab 20 Thermo Fischer, Espoo, Finland) [8 (link)]. HbA1c was analyzed directly using ion exchange high-performance liquid chromatography (HPLC) with commercially available reagents (D-10 Hemoglobin A1c Program #12000949) in a fully automated system (D-10 Hemoglobin Testing System #12010405, Bio-Rad, Hercules, CA, USA). Fasting serum insulin levels were analyzed using a Luminex multiplex system (Luminex Corp, Austin, TX, USA) and a commercially available kit, catalog no. HINS-MAG. The intra-assay % CV was <10 and the inter-assay % CV was <15, according to the manufacturer’s protocol.

Serum samples from the chosen patients were allowed to defrost prior to biochemical analysis. HbA1c was analyzed directly using ion-exchange high-performance liquid chromatography (HPLC) with commercially available reagents (D-10 Hemoglobin A1c Program #12000949) in a fully automated system (D-10 Hemoglobin Testing System #12010405, Bio-Rad, Hercules, CA, USA). Fasting serum insulin levels were analyzed using a Luminex multiplex system (Luminex Corp, Austin, TX, USA) and a commercially available kit, catalog no. HINS-MAG. The intra-assay % CV was <10 and the inter-assay % CV was <15, according to the manufacturer’s protocol. Serum glucose, triglyceride, cholesterol and high-density lipoprotein (HDL) levels were analyzed regularly using commercially purchased kits (catalog nos. 981812, 981379, 981823 and 981301, respectively) and an automated biochemical analyzer (Konelab 20 Thermo Fischer, Espoo, Finland), as we described previously [23 (link)].