The miR-106a mimics, miR-106a inhibitor, ATG7 siRNA, ATG16L1 siRNA, and ULK1 siRNA were purchased from GenePharma biotechnology company. To assay luciferase activity, HEK 293T cells were cotransfected with the pmirGLO luciferase constructs (WT or Mut) and miR-106a mimics or miR-106a inhibitor using Lipofectamine 2000 according to the manufacturer’s instruction. For autophagy analysis, THP-1 macrophages were transfected with 50 nM mimic negative control (mimic nc) or miR-106a mimics; inhibitor negative control (inhibitor nc) or miR-106a inhibitor; 50 pmol ATG7 siRNA, ATG16L1 siRNA, or ULK1 siRNA according to the manufacturer’s instructions. Several chemical reagents were also used to treat THP-1 macrophages: a lysosome inhibitor, bafilomycin A1 (100 nM; Baf A1, Selleck); An autophagy inducer, rapamycin (50 μg/ml; Rapa, Solarbio Science & Technology Co.).
Ulk1 sirna
Manufactured by GenePharma
ULK1 siRNA is a small interfering RNA (siRNA) designed to target the ULK1 gene, which is involved in the regulation of autophagy, a cellular process that helps recycle damaged or unnecessary components. The core function of ULK1 siRNA is to knockdown the expression of the ULK1 gene, thereby modulating the autophagy pathway. This product is intended for research use only and its specific applications should be determined by the user.
Automatically generated - may contain errors
2 protocols using ulk1 sirna
1
Autophagy Regulation by miR-106a in Macrophages
2
Knockdown of ULK1 in Keratinocytes
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To knock down ULK1, ULK1-siRNA or non-coding siRNA (NC-siRNA) (GenePharma, Shanghaia) was transfected with Lipofectamine RNAiMax (Thermo Fisher Scientific, Waltham) into keratinocytes at a concentration of 60nM.
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