Single cells were isolated and labeled with a fixable viability dye and antibodies to IgA-FITC (BD Biosciences, #553478); IgA-PE (BD Biosciences, #562141); CD69-BV786 (BD Biosciences, #56468); B220-BV605 (BD Biosciences, #563708); CD19-PerCp-Cy5.5 (BD Biosciences, #551001); CD11c-PE-CY7 (BD Biosciences, #558079); CD317 (pDCA1)-BV421 (BD Biosciences, #566431); CD268 (BAFF Receptor)-APC (eBioscience, #17-9117-42); and LIVE/DEAD™ Near IR- Dead Cell Stain Kit (Molecular Probes, #L34975). Isotype controls were used to set the photomultiplier tube (PMT) voltages. Single color controls were used for compensation. Stained cells were assayed on the LSRFortessa™ cell analyzer (BD Biosciences), and flow data were analyzed and plotted using BD FACSDiva™ (ver. 8). Singlet live cells were gated before further analysis (Supplementary Fig. 1 ).
Cd69 bv786
Manufactured by BD
CD69-BV786 is a fluorochrome-conjugated antibody that binds to the CD69 cell surface antigen. CD69 is an early activation marker expressed on various immune cell types. The BV786 fluorochrome is a violet-excited, far-red emitting dye that can be used in multicolor flow cytometry panels.
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Lab products found in correlation
Cd19 percp cy5, by BD (1 mentions)
Iga pe, by BD (1 mentions)
B220 bv605, by BD (1 mentions)
Cd11c pe cy7, by BD (1 mentions)
Lsrfortessa cell analyzer, by BD (1 mentions)
Facsdiva, by BD (1 mentions)
Iga fitc, by BD (1 mentions)
Cd45ra bv650, by BD (1 mentions)
Cd45ro pe cf594, by BD (1 mentions)
Cd4 buv395, by BD (1 mentions)
Cd8 apc h7, by BD (1 mentions)
Cd25 pe cy7, by BD (1 mentions)
Cd27 buv737, by BD (1 mentions)
Cd279 pd 1 bv421, by BD (1 mentions)
Cd197 ccr7 pe, by BD (1 mentions)
Brilliant stain buffer plus, by BD (1 mentions)
2 protocols using cd69 bv786
1
Multiparameter Flow Cytometry Analysis
2
Comprehensive T Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A multi-color flow cytometry panel was designed to assess T cell phenotype markers. At select time points during expansion, T cells were stained using an antibody cocktail (CD4-BUV395, CD8-APC-H7, CD25-PE-Cy7, CD27-BUV737, CD45RA-BV650, CD45RO-PE-CF594, CD69-BV786, CD197/CCR7-PE, and CD279/PD1-BV421; BD Biosciences) diluted in staining solution (1:1 mixture of Brilliant Stain Buffer Plus [BD Biosciences, #566385] and PBS with 1% FBS, 10 mM HEPES, and 2 mM EDTA) for 30 min at room temperature, centrifuged, re-suspended in fixation solution (1% formaldehyde in PBS), and immediately analyzed.
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