Hmgcs1

Manufactured by Cell Signaling Technology

HMGCS1 is an enzyme that catalyzes a key step in the mevalonate pathway, which is essential for the biosynthesis of cholesterol and other isoprenoid compounds. It is a multi-domain protein that performs the rate-limiting step in this metabolic pathway.

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Lab products found in correlation

2 protocols using hmgcs1

Immunostaining and Western Blot Antibodies

Cited 1 time

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Antibodies used for immunostaining: rabbit- anti Olig2 (EMD Millipore AB9610, 1:500), mouse-anti APC [CC1] (EMD Millipore OP80, 1:500), mouse-anti Ki67 (BD Pharmingen 550609, 1:50), donkey anti-rabbit IgG (H + L) secondary- Alexa Fluor 568 (Thermo Fisher Scientific A10042, 1:300) and donkey anti-mouse IgG (H + L) secondary- Alexa Fluor 488 (Thermo Fisher Scientific A21202, 1:300). These antibodies have been documented in a previous porcine study (Ishibashi et al., 2012 (link)).
Antibodies used for western blots: rabbit-anti MBP (Cell signaling 78896, 1:1,000), rabbit-anti PLP1 (Cell Signaling Technology 85971S, 1:1,000), rabbit-anti HMGCS1 (Cell Signaling Technology 42201S, 1:1,000), rabbit-anti GAPDH (Cell Signaling Technology 5174, 1:1,000), mouse anti-β-Actin C4 (Santa Cruz 47778, 1:1,000), mouse anti-MAG (Santa Cruz 166849, 1:1,000), goat anti-MOG (Novus Biologicals NB300–948, 1:1,000), goat anti-rabbit IgG (H+L)-HRP (Thermo Fisher Scientific G21234, 1:5,000), goat anti-mouse IgG (H+L)-HRP (Thermo Fisher Scientific G16072, 1:5,000), donkey anti-goat IgG (H+L)-HRP (Thermo Fisher Scientific A15999, 1:5,000). Gray matter tissue was used as a negative control.

Ahmed S., Travis S.D., Díaz-Bahamonde F.V., Porter D.D., Henry S.N., Mykins J., Ravipati A., Booker A., Ju J., Ding H., Ramesh A.K., Pickrell A.M., Wang M., LaConte S., Howell B.R., Yuan L, & Morton P.D. (2021). Early Influences of Microbiota on White Matter Development in Germ-Free Piglets. Frontiers in Cellular Neuroscience, 15, 807170.

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Western Blot Analysis of Prostate Cancer Markers

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Lysis buffer contained 50 mM Tris-HCl, 150 mM NaCl, 1% NP40, 10 mM NaF, 10% Glycerol, and protease inhibitor cocktail (Roche, ON, Canada. Cat. No. 04693116001). Western blots were imaged by a LI-COR Odyssey system. Loading controls were run on the same blots. Primary antibodies include: AR (Santa Cruz Biotechnology, TX, USA. Cat. No. sc-816), PSA (Cell Signaling Technology, MA, USA. Cat. No. 5877), SEMA3C (Santa Cruz Biotechnology Cat. No. sc-27796), AKR1C3 (Abcam, ON, Canada. Cat. No. ab49680), HSD3B2 (Abnova, ON, Canada. Cat. No. 3284-mo2), HSD17B3 (Abnova Cat. No. 29–119), FASN (Cell Signaling Technology. Cat. No. 3180), HMGCS1 (Cell Signaling Technology. Cat. No. 36877), SREBP-1 (Santa Cruz Biotechnology. Cat. No. sc-13551), UGT2B17 (Abcam. Cat. No. ab126269) and actin (Sigma-Aldrich, ON, Canada. Cat. No. A2066). Secondary antibodies were anti-rabbit alexa fluor 680 (Invitrogen, Cat. No. A21109), anti-mouse alexa fluor 680 (Invitrogen, Cat. No. A21058), anti-goat alexa fluor 680 (Invitrogen, Cat. No. A21084).

Battin T.J., Wille A., Sattler B, & Psenner R. (2001). Phylogenetic and Functional Heterogeneity of Sediment Biofilms along Environmental Gradients in a Glacial Stream. Applied and Environmental Microbiology, 67(2), 799-807.

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