Mfp 3d bio model

Manufactured by Oxford Instruments

Sourced in United States

The MFP-3D-Bio is an atomic force microscope (AFM) designed for biological and life science applications. It provides high-resolution imaging and analysis capabilities for studying the surface topology and properties of biological samples. The instrument features a closed-loop scanner and advanced vibration isolation for stable and accurate measurements.

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Lab products found in correlation

Model ix81, by Olympus (2 mentions) Co2 independent medium, by Thermo Fisher Scientific (1 mentions) Mlct o10, by Bruker (1 mentions) Igor pro software, by Wavemetrics (1 mentions)

Spelling variants of this product

MFP-3D (194 citations) MFP-3D-BIO (62 citations)

4 protocols using mfp 3d bio model

Atomic Force Microscopy of Extracellular Vesicles

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Vesicles were adhered to freshly cleaved mica by incubation at room temperature for 15 minutes followed by washing [44 ]. Atomic force microscopy was performed using an MFP-3D-Bio model (Asylum Research) with a pyramidal tip (Bruker; MLCT, triangular, resonant frequency: ~125 kHz) as described previously [30 (link)]. Briefly, vesicles with a size range between 50~300 nm were found by scanning in a tapping (AC) mode and indented until reaching 0.5 nN at 250 nm/s to generate a force-displacement curve. The data were analysed and converted to Young’s modulus (E) using MATLAB by modeling EVs as thin elastic shells [46 (link)]. The slope of the approach curve was calculated over a sliding interval and the surface of the vesicle was determined by a high and sustained change in slope. The linear region was used to calculate E via the equation

F (δ) = \frac{a E t^{2}}{r} δ

With F as measured cantilever force and δ as tip displacement. The constant

\frac{a t^{2}}{r}

is determined by vesicle geometry and assumed to be ~0.87nm.

Lenzini S., Bargi R., Chung G, & Shin J.W. (2020). Matrix mechanics and water permeation regulate extracellular vesicle transport. Nature nanotechnology, 15(3), 217-223.

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Atomic Force Microscopy of Extracellular Vesicles

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F (δ) = \frac{a E t^{2}}{r} δ

With F as measured cantilever force and δ as tip displacement. The constant

\frac{a t^{2}}{r}

is determined by vesicle geometry and assumed to be ~0.87nm.

Lenzini S., Bargi R., Chung G, & Shin J.W. (2020). Matrix mechanics and water permeation regulate extracellular vesicle transport. Nature nanotechnology, 15(3), 217-223.

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Real-time Nanomechanical Characterization of VSMCs

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Real-time monitoring of biomechanical properties of live VSMC was performed using an Asylum AFM System (Model MFP-3D-BIO, Asylum Research, Santa Barbara, CA) mounted on an inverted microscope (Model IX81, Olympus America Inc.). A 5 μm diameter glass microbead was glued to an AFM probe (MLCT-O10, Santa Barbara, CA; Bruker Corp.) and used for E-modulus measurement. All AFM measurements were conducted at room temperature (~25 °C) in CO₂ independent medium (Invitrogen) without antibiotics. The parameters employed were a 0.5 Hz sampling frequency, with an approach/retraction velocity of 1 µm/sec, 1000 nm traveling distance for one sampling cycle (indentation and retraction), and approximately 1000–3000 pN loading force resulting in an average cellular indentation of 200 nm. Cells were randomly selected and indented at a site between the nucleus and cell boundary to collect approximately 900 force curves within 30 min. To minimize drifting, after the probe was submerged in cell bath, the AFM system was thermally and mechanically equilibrated for at least 30 min. Each cantilever was calibrated after a given adhesion and before a stiffness experiment using thermal noise amplitude analysis^{31 (link),32}.

Sanyour H., Childs J., Meininger G.A, & Hong Z. (2018). Spontaneous oscillation in cell adhesion and stiffness measured using atomic force microscopy. Scientific Reports, 8, 2899.

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Cell-Cell Adhesion Measurements via AFM

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All AFM experiments including measurements of cell-cell adhesion were performed using an Asylum Research AFM System (Model MFP-3D-BIO, Asylum Research, Santa Barbara, CA) with IGOR Pro software (WaveMetrics Inc., Oregon). The system was mounted on an inverted optical microscope (Model IX81, Olympus America Inc.).

Xie L., Sun Z., Hong Z., Brown N.J., Glinskii O.V., Rittenhouse-Olson K., Meininger G.A, & Glinsky V.V. (2018). Temporal and molecular dynamics of human metastatic breast carcinoma cell adhesive interactions with human bone marrow endothelium analyzed by single-cell force spectroscopy. PLoS ONE, 13(9), e0204418.

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