Pe cy7 conjugated cd45 antibody

To evaluate the engraftment and multi-lineage differentiation of the human CD34+ cells transduced with the hAS8 lentiviral vector, cells from the peripheral blood, bone marrow, thymus and spleen were analyzed from the transplanted NRG mice. Total cells were obtained from the above-mentioned organs and were labeled with antibodies specific for human immune cells including T cells, B cells, macrophages and CD34+ cells. The following antibodies were used in these studies: T cell antibodies used were a Brilliant violet (BV) 421-conjugated CD3 antibody, an allophycocyanin (APC) H7-conjugated CD4 antibody and an Alexa fluor (AF) 700-conjugated CD8 antibody (BD Biosciences, San Jose, CA). B cell antibodies used were a PE-CY7-conjugated CD45 antibody and a PE-conjugated CD19 antibody (BD Biosciences, San Jose, CA). Macrophage antibodies used were a PE-CY7-conjugated CD45 antibody and a PE-conjugated CD14 antibody (BD Biosciences). CD34+ cell antibodies used were a PE-CY7-conjugated CD45 antibody and a PE-conjugated CD34 antibody (BD Biosciences, San Jose, CA). Flow cytometry was performed using a BD Fortessa and analyzed with CXP software.