Recombinant lentiviruses targeting H19 (shH19-1 and shH19-2) and Lenti-shNC were purchased from GenePharma Company (Shanghai, China). hDPSCs were transfected by lentiviruses exposure in 1 mL α-MEM supplemented with 10% FBS and 5 μg/mL polybrene for 24 h. H19 overexpression plasmid pcDNA3.1-H19, miR-140-5p mimics, and scramble control (NC) were chemically synthesized by GenePharma. When hDPSCs were 70–80% confluent, pcDNA-H19 and miRNA mimic transfection was performed using Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer’s instructions. qRT-PCR analysis was used to detect H19 and miR-140-5p expression levels to validate the transfection efficiencies. The cells were cultured in mineralizing medium for odontoblastic differentiation 48 h after transfection.
Lenti shnc
Manufactured by GenePharma
Sourced in China
Lenti-shNC is a lentiviral vector system used for gene silencing in cell lines and primary cells. It contains a non-targeting shRNA sequence designed for use as a negative control.
Automatically generated - may contain errors
Lab products found in correlation
Mir 140 5p mimic, by GenePharma (3 mentions)
Pcdna3.1 h19, by GenePharma (3 mentions)
Shh19 1, by GenePharma (3 mentions)
Shh19 2, by GenePharma (3 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions)
Scramble control nc, by GenePharma (2 mentions)
α mem, by GenePharma (1 mentions)
5 protocols using lenti shnc
1
Lentiviral-mediated Modulation of H19 and miR-140-5p in hDPSCs
2
Lentiviral-mediated H19 Gene Manipulation
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Recombinant lentiviruses containing full-length H19 (Gene Bank accession number, NR_002196.1) and scramble control (NC) were obtained from GenePharma Company (Shanghai, China). Recombinant lentiviruses targeting H19 (Lenti-shH19-1 and Lenti-shH19-2) and scramble control (Lenti-shNC) were also obtained from GenePharma Company. SCAPs were transfected by lentiviruses exposure in 1 mL α-MEM supplemented with 10% FBS and 8 μg /mL polybrene (POL) for 10 h. Infected cells were cultured in the conventional medium and the expression level of H19 was detected.
3
Lentivirus-Mediated Gene Modulation in HAMSCs
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Recombinant lentiviruses containing full-length H19 (Gene Bank accession number, NR_002196.1) and scramble control (NC) were obtained from Integrated Biotech Solutions Company (Shanghai, China) and were named Lenti-H19 and Lenti-NC. Recombinant lentiviruses targeting H19 and scramble control named Lenti-shH19 and Lenti-shNC, respectively, were obtained from GenePharma Company (Shanghai, China). All lentivirus vectors contained the green fluorescent reporter gene (GFP). The viruses were used to infect HAMSCs and establish stably expressing transfectants. HAMSCs were exposed to viral supernatant containing 1 mL DMEM supplemented with 10% FBS and 8 μg /mL polybrene (POL) for 10 h.
4
Lentiviral-mediated H19 Regulation in hDPSCs
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Recombinant lentiviruses targeting H19 (shH19-1 and shH19-2) and Lenti-shNC were purchased from GenePharma Company (Shanghai, China). hDPSCs were transfected by lentiviruses exposure in 1 mL α-MEM supplemented with 10% FBS and 5μg /mL polybrene for 24 h. H19 overexpression plasmid pcDNA3.1-H19, miR-140-5p mimics and and scramble control (NC) were chemically synthesized by GenePharma. When hDPSCs were 70%-80% con uent, pcDNA-H19 and miRNA mimic transfection was performed using Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer's instructions. qRT-PCR analysis was used to detect H19 and miR-140-5p expression levels to validate the transfection e ciencies. The cells were cultured in mineralizing medium for odontoblastic differentiation 48 h after transfection.
5
Modulating H19 and miR-140-5p in hDPSCs
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+ Expand
Recombinant lentiviruses targeting H19 (shH19-1 and shH19-2) and Lenti-shNC were purchased from GenePharma Company (Shanghai, China). hDPSCs were transfected by lentiviruses exposure in 1 mL α-MEM supplemented with 10% FBS and 5 µg /mL polybrene for 24 h. H19 overexpression plasmid pcDNA3.1-H19, miR-140-5p mimics and and scramble control (NC) were chemically synthesized by GenePharma. When hDPSCs were 70%-80% con uent, pcDNA-H19 and miRNA mimic transfection was performed using Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer's instructions. qRT-PCR analysis was used to detect H19 and miR-140-5p expression levels to validate the transfection e ciencies. The cells were cultured in mineralizing medium for odontoblastic differentiation 48 h after transfection.
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