PIK3IP1-specific shRNA was obtained from Corues Biotechnology. For lentivirus production, HEK-293T cells were transfected with PIK3IP1 shRNA, psPAX2, and pMD2.G using the ExFect transfection reagent T101 according to the lipofectamine manual (Vazyme, Nanjing, China). MV4-11 or U937 cells were infected with a viral supernatant containing 8 μg/mL of polybrene for 72 h. The pLV3ltr-ZsGreen-puro-U6 vector was used as the vehicle control. Cells were selected with puromycin for 7 days followed by puromycin maintenance. The infection efficiency was assessed by western blot and RT-PCR.
Exfect transfection reagent t101
Manufactured by Vazyme
Sourced in China
ExFect transfection reagent T101 is a cationic lipid-based transfection reagent designed for efficient delivery of nucleic acids into a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using exfect transfection reagent t101
1
Targeted PIK3IP1 Knockdown via Lentiviral Transduction
2
Lentiviral Knockdown of c-MYC in U937 Cells
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Corues Biotechnology provided c-MYC-specific shRNA and control lentiviral particles. For lentiviral transduction, we inoculated 10,000 cells per well in 6-well tissue culture plates and infected them with lentiviral particles using the ExFect transfection reagent T101 for 48 h, following the instructions mentioned in the lipofectamine manual (Vazyme, Nanjing, China). After infection, U937 cells were maintained for 7–10 days and selected with 8 μg/mL puromycin. We determined the infection efficiency by analyzing the expression levels of c-MYC using RT-qPCR and western blot.
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