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U87mg

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The U87MG is a commonly used cell line derived from a human glioblastoma, a type of brain cancer. It is a well-established model system for research purposes, particularly in the fields of oncology and neuroscience. The U87MG cell line serves as a standardized tool for studying various aspects of glioblastoma biology, including cell proliferation, migration, and response to treatments.

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Lab products found in correlation

U87mg, by American Type Culture Collection (2 mentions) Ciprofloxacin, by Santa Cruz Biotechnology (1 mentions) Lenti x 293t, by Thermo Fisher Scientific (1 mentions) Panc 1, by GE Healthcare (1 mentions) Huh 7, by GE Healthcare (1 mentions) Lenti x 293t, by GE Healthcare (1 mentions) Penicillin streptomycin, by Welgene (1 mentions) Heparin sulfate, by Merck Group (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Ln229, by American Type Culture Collection (1 mentions) Hyclone, by GE Healthcare (1 mentions)

3 protocols using u87mg

1

Cultivation of Various Cell Lines

Cited 3 times
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The human embryonic kidney cell line 293T and the U87MG human glioma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). 293FT and Lenti-X 293T cells were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Takara Bio Inc. (Shiga, Japan), respectively. The A549 human non-small cell lung cancer cell line, PANC-1 human pancreatic cancer cell line, NCI-H1299 human metastatic lung cancer cell line, Huh7 human hepatocellular carcinoma cell line, and CSC2 human glioma stem cell line were obtained from various researchers as described previously17 (link)–21 (link). The 293T, 293FT, Lenti-X 293T, U87MG, PANC-1, and Huh7 cells were cultured in Dulbecco’s modified Eagle medium (DMEM; HyClone - GE Healthcare, Chicago, IL, USA). The A549 and NCI-H1299 cells were cultured in RPMI 1640 medium (HyClone). The CSC2 glioma stem cells were cultured as described previously20 (link). All media except that used to culture CSC2 were supplemented with 10% fetal bovine serum (HyClone), 1% penicillin/streptomycin (Welgene, Gyeongsan, Republic of Korea), and 10 μg/ml ciprofloxacin (Santa Cruz Biotech, Santa Cruz, CA, USA).
Seo Y., Kim S.S., Kim N., Cho S., Park J.B, & Kim J.H. (2020). Development of a miRNA-controlled dual-sensing system and its application for targeting miR-21 signaling in tumorigenesis. Experimental & Molecular Medicine, 52(12), 1989-2004.
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2

Culturing Glioblastoma Cell Lines

Cited 1 time
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The human adherent GBM cell lines, U-87 MG and LN-229, were obtained from the American Type Culture Collection (ATCC) and were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen™, Life technologies, New York) supplemented with fetal bovine serum (FBS; 10% for U-87 MG and 5% for LN-229; GE Healthcare Life Sciences, Utah). The adult human GBM neurosphere cell lines, GBM14, GBM10, JHH520, and HSR-GBM1 were cultured in neurosphere media containing human epidermal growth factor (EGF; PeproTech, New Jersey), fibroblast growth factor (FGF; PeproTech, New Jersey) and heparin sulfate (Sigma-Aldrich, Missouri) as previously described [45 (link), 46 (link)]. HEK293T and HEK293GP cells were used for producing viral particles and were cultured in DMEM containing 10% FBS. All these cell lines were cultured in a humidified atmosphere of 5% CO2 at 37°C.
Kaur H., Ali S.Z., Huey L., Hütt-Cabezas M., Taylor I., Mao X.G., Weingart M., Chu Q., Rodriguez F.J., Eberhart C.G, & Raabe E.H. (2016). The transcriptional modulator HMGA2 promotes stemness and tumorigenicity in glioblastoma. Cancer letters, 377(1), 55-64.
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Glioblastoma Cell Lines and Astrocytes

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All 60 tissue samples were collected from Harbin Medical University (Heilongjiang, China). The present study received ethical approval from Harbin Medical University. The clinical characters of these samples were listed in Table I. Written informed consent approving this study was obtained from each patient. Human glioblastoma cell lines U-87MG (www.atcc.org/Products/All/HTB-14.aspx) and U251 were from the American Tissue Culture Collection (ATCC, Manassas, VA, USA). The U-87MG cell line is known to be cross-contaminated with another cell line of unknown origin, but it is likely to be another glioblastoma cell line (20 (link)). U-87MG cells were grown in Minimum Essential Medium (HyClone; GE Healthcare Life Sciences, Logan, UT, USA) with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). U251 cells were maintained in Dulbecco's High Glucose Modified Eagle Medium (HyClone; GE Healthcare Life Sciences) with 10% FBS. Normal human astrocytes (NHA) obtained from Lonza (www.lonza.com/products-services/bio-research/primary-cells/human-cells-and-media/neural-cells-and-media/nha-normal-human-astrocytes.aspx) were cultured in the provided astrocyte growth media and 5% FBS. Cells were incubated in a humidified atmosphere with 5% CO2 at 37°C.
Liu Z., Su D., Qi X, & Ma J. (2018). MiR-500a-5p promotes glioblastoma cell proliferation, migration and invasion by targeting chromodomain helicase DNA binding protein 5. Molecular Medicine Reports, 18(3), 2689-2696.
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