Universal mycoplasma detection kit atcc 30 1012 k

Human cancer and normal cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and were tested negatively for mycoplasma using Universal Mycoplasma Detection Kit—ATCC-30-1012 K (ATCC). H460 cells (non-small cell lung carcinoma) were cultured in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA). HCT116 cells (colorectal carcinoma) were cultured in McCoy’s 5A medium (Sigma-Aldrich, St. Louis, MO, USA). Both media contained: 10% fetal bovine serum (FBS; Biowest, Riverside, MO, USA), 100 µg·mL⁻¹ of streptomycin, and 100 unit·mL⁻¹ of penicillin. MRC-5 and CCD841CoN normal cells were cultured in EMEM medium (Eagle’s Minimal Essential Medium, Sigma-Aldrich, St. Louis, MO, USA). supplemented with 10% FBS, without antibiotics. All cells were incubated in a humidified atmosphere containing 5% CO₂ at 37 °C. Experiments were performed with cells in the exponential phase of growth.

The human non-small-cell lung cancer (H460), human prostate cancer (Du-145 and LNCaP), and human fetal lung fibroblasts (MRC-5) cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The human prostatic PNT1A cell line was kindly provided by Prof. Jędrzej Antosiewicz (Medical University of Gdańsk, Gdańsk, Poland). Cells were routinely tested for mycoplasma (Universal Mycoplasma Detection Kit—ATCC-30-1012 K; ATCC; Manassas, VA, USA) and found to be negative. H460, LNCaP, and PNT1A cells were cultured in RPMI 1640 medium (Sigma-Aldrich, USA). Du-145 and MRC-5 cells were grown in an EMEM medium (Eagle’s Minimal Essential Medium; Sigma-Aldrich, USA). Both media were supplemented with 10% FBS (fetal bovine serum; Biowest, Riverside, MO, USA), 100 µg·mL⁻¹ of streptomycin (Sigma-Aldrich, Shanghai, China), and 100 unit·mL⁻¹ of penicillin (Sigma-Aldrich, Rehovot, Israel). MRC-5 cells were cultured in an EMEM medium with 10% FBS without antibiotics. All cells were incubated in a humidified atmosphere containing 5% CO₂ at 37 °C. Experiments were performed with cells in the exponential phase of growth.

Human cancer (H460, Du-145, and LNCaP) and normal (MRC-5) cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The PNT1A normal cell line was provided by Prof. Jędrzej Antosiewicz from the Medical University of Gdańsk (Gdańsk, Poland). Cell lines were tested negatively for mycoplasma using a Universal Mycoplasma Detection Kit—ATCC-30-1012 K (ATCC). H460, LNCaP, and PNT1A cells were cultured in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA). Du-145 and MRC-5 cells were cultured in EMEM medium (Eagle’s Minimal Essential Medium, Sigma-Aldrich, St. Louis, MO, USA). Both media contained 10% fetal bovine serum (FBS; Biowest, Riverside, MO, USA), 100 µg × mL⁻¹ of streptomycin, and 100 unit × mL⁻¹ of penicillin. In the case of MRC-5 cells, the medium was supplemented only with 10% FBS, without antibiotics. All cells were incubated in a humidified atmosphere containing 5% CO₂ at 37 °C. Experiments were performed with cells in the exponential phase of growth.