Anti cd8α clone yts 169

NTS model: Mice were injected with rabbit IgG antibody in complete Freud’s Adjuvant s.c. in the footpad. Depending on the experiment setup, three or five days later, NTS was induced by i.v. injection with nephrotoxic rabbit anti-mouse serum [30 (link)]. Mice were kept for 7 days.
Injections: Low-dose rIL-15 was administered via a single i.p. injection in a 0.1 µg/g bodyweight concentration [31 (link),32 (link),33 (link)]. Anti-CD8α (Clone YTS 169.4, BioXCell, Lebanon, NH, USA) depletion antibody and respective isotype control (Clone LTF-2, BioXCell, Lebanon, NH, USA) were injected i.p. in a concentration of 200 µg/20 g bodyweight twice per week.

Isotype control (2A3, catalogue number BE0089), anti-PD-L1 (clone 10 F.9G2, catalogue number BE0101) and anti-CTLA-4 (9H10, catalogue number BE0131) were purchased from BioXcell. Mice were treated every 4 days for up to 2 doses. Where indicated mice were treated at days 14, 16, 18 and 20 post-tumor inoculation with 25 µg FTY720 (Sigma). Anti-asialo GM-1 (catalogue number 986-10001, FUJIFILM Wako Pure Chemical Corporation) and anti-CD8α (clone YTS 169.4, catalogue number BE0117, BioXcell) were used for in vivo NK cell and CD8⁺ T cell depletion, respectively. Anti-CD3 (clone 145-2C11) and anti-CD28 (clone 37.51) used to stimulate murine T cells were obtained from BD Pharmingen. Cytokines used for the stimulation of murine or Human immune cells were purchased from Biolegend.