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2 protocols using reduced form of β nicotinamide adenine dinu cleotide phosphate β nadph

1

Comprehensive Metabolic Enzyme Assay

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Chrysin, caffeine, paraxanthine, theobromine, theophylline, ethoxyresorufin, methoxyresorufin, benzyloxyresorufin, resorufin, p-nitrophenol, erythromycin, 4-dimethylaminoantipyrine, glucose 6-phosphate, glucose-6-phosphate dehydrogenase, β-glucuronidase (type H-3 from Helix pomatia), sulfatase (type H-1 from Helix pomatia) and D-saccharic acid 1,4-lactone monohydrate were obtained from Sigma-Aldrich (St. Louis, MO, USA). The reduced form of β-nicotinamide adenine dinu-cleotide phosphate (β-NADPH) was purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan), and methanol and acetonitrile were HPLC-grade from J.T.Baker (Center Valley, PA, USA). All other chemicals were of analytical grade and used as received.
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2

Baicalin Phytochemical Analysis Protocol

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Baicalin (purity, >90.0%) was supplied form Tokyo Chemical Industry. Baicalein, caffeine, paraxanthine, theobromine, theophylline, ethoxyresorufin, methoxyresorufin, benzyloxyresorufin, resorufin, p-nitrophenol, erythromycin, glucose-6-phosphate and glucose-6-phosphate dehydrogenase were obtained from Sigma (St. Louis, MO, USA). The reduced form of β-nicotinamide adenine dinucleotide phosphate (β-NADPH) was purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan), and methanol and acetonitrile were HPLC-grade from J.T. Baker (Central Valley, PA, USA). All other chemicals were of analytical grade and used as received.
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