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2 9 dimethyl 1 10 phenanthroline neocuproine

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, Germany

2,9-Dimethyl-1,10-phenanthroline (neocuproine) is a chemical compound used as a chelating agent in analytical chemistry and biochemistry. It forms stable complexes with copper(I) ions, which can be used for the detection and quantification of copper in various samples.

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2 protocols using 2 9 dimethyl 1 10 phenanthroline neocuproine

1

Antioxidant Capacity Assessment Protocol

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L-Carnosine, aminoguanidine HCl, sodium azide, nitro blue tetrazolium (NBT), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), copper (II) chloride, sorbitol, ammonium acetate, α-crystallin from bovine eye lens, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich, UK. Ascorbic acid, glutathione, and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from TCI, Toshima, Tokyo, Japan. 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) was purchased from Acros Organics, New Jersey, USA. 2,9-Dimethyl-1,10-phenanthroline (neocuproine) and 5,5′-dithiobis (2-nitrobenzoic acid) (Ellman's reagent) were purchased from Alfa Aesar, Heysham, UK. All other chemicals and reagents were of analytical grade and used as received.
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2

Reagents for Cellular and Biochemical Assays

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Bicinchoninic acid (BCA), D-glucose, Drabkin’s reagent, ferrous sulfate (FeSO4), fluorescein isothiocyanate-dextran (FITCDextran), formic acid, mannitol and PKH67 (green fluorescent cell linker) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), L-glutamine, penicillin/streptomycin, amphotericin B and HAT (hypoxanthine/aminopterin/thymidine) were obtained from Pan Biotech (Aidenbach, Germany). 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (ferrozine) and 2,9-Dimethyl-1,10-phenanthroline(neocuproine) were purchased from Alfa Aesar (Kandel, Germany). MitoSOX Red mitochondrial superoxide indicator and pHrodo Red were purchased from Thermo Fisher (Carlsbad, CA, USA).
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