Dmem liquid media

Dulbecco’s Modified Eagle Medium (DMEM) powder was purchased from Gibco by Life Technology (Thermo Fisher Scientific, Waltham, MA, USA). Calcium chloride dihydrate (CaCl₂·2H₂O), sodium bicarbonate (NaHCO₃), sodium hydrogen phosphate (Na₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), sodium citrate tribasic dihydrate, sodium succinatedibasic hexahydrate and trypsin-ethylene diaminetetraacetate (EDTA) salts were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dimethyl sulphoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), nonidet P40 substitute (NP40) and anti-cancer drug DOX were acquired from Sigma-Aldrich (St. Louis, MO, USA). DMEM liquid media, foetal bovine serum (FBS), TrypLE Express, and penicillin-streptomycin were procured from Sigma-Aldrich (St. Louis, MO, USA). Sodium chloride and potassium chloride salts were bought from Fischer Scientific (Loughborough, UK). Acetonitrile (ACN), hydrochloric acid (HCl), methanol and glycerol were from Fischer Scientific (Loughborough, UK). DOX was dissolved in distilled water and 8.62 mM stock solution was prepared.

Dulbecco’s modified Eagle medium (DMEM) powder was purchased from Gibco by Life Technology (Thermo Fisher Scientific, USA). Calcium chloride dihydrate (CaCl₂∙2H₂O), sodium bicarbonate (NaHCO₃), sodium hydrogen phosphate (Na₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), sodium citrate tribasic dihydrate, and alpha-ketoglutaric acid disodium salt hydrate were obtained from Sigma-Aldrich (St Louis, MO, USA). Anticancer drugs DOX and CYP were acquired from Sigma-Aldrich (St Louis, MO, USA). DMEM liquid media, fetal bovine serum (FBS), trypsin, TrypLE Express, penicillin–streptomycin, and trypan blue were procured from Sigma-Aldrich (St Louis, MO, USA). Sodium chloride and potassium chloride salts were bought from Fischer Scientific (Loughborough, UK). Acetonitrile (ACN), hydrochloric acid (HCl), and methanol were from Fischer Scientific (Loughborough, UK). Ammonium bicarbonate, formic acid, dithiothreitol (DTT), trifluoroacetic (TFA), trifluoroethanol (TFE) acid, and iodoacetamide (IAM) were from Sigma-Aldrich (St. Louis, MO, USA).