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Smz745 zoom

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The SMZ745 zoom is a stereo microscope with a magnification range of 0.67x to 5.0x. It features a working distance of 116mm and a maximum field of view of 35mm. The microscope is designed for detailed observation and analysis of samples.

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SMZ745 (81 citations)

2 protocols using smz745 zoom

1

RNAi Screening of C. elegans Genes

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To perform RNAi, unc-22 (Kamath et al., 2001 ) or lin-1 (Kamath et al., 2003 (link)) or empty L4440 plasmids were transformed into E. coli HT115 bacteria and were cultured overnight at 37°C in LB media containing 100 µg/ml ampicillin. The cultured bacteria were seeded onto C. elegans growth media plates (NGM) containing 100 µg/ml ampicillin and 10 µg/ml IPTG and incubated overnight at RT overnight. Synchronized embryos were placed on the plates and incubated for 72–96 hours at 20°C before scoring. Worms were then scored using Nikon SMZ745 zoom stereomicroscope for PVL, BOW, or Multivulva phenotype. Worms presenting the phenotypes were counted in relative to the total number of worm in each plate. In the RNAi experiments the fraction of worms presenting the phenotype in the empty vector experiment was subtracted from the fraction of worms presenting the phenotype in the unc-22 or lin-1 RNAi experiments.
Ganem N.S., Ben-Asher N., Manning A.C., Deffit S.N., Washburn M.C., Wheeler E.C., Yeo G.W., Ben-Naim Zgayer O., Mantsur E., Hundley H.A, & Lamm A.T. (2019). Disruption in A-to-I Editing Levels Affects C. elegans Development More Than a Complete Lack of Editing. Cell reports, 27(4), 1244-1253.e4.
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2

RNAi Screening of C. elegans Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
To perform RNAi, unc-22 (Kamath et al., 2001 ) or lin-1 (Kamath et al., 2003 (link)) or empty L4440 plasmids were transformed into E. coli HT115 bacteria and were cultured overnight at 37°C in LB media containing 100 µg/ml ampicillin. The cultured bacteria were seeded onto C. elegans growth media plates (NGM) containing 100 µg/ml ampicillin and 10 µg/ml IPTG and incubated overnight at RT overnight. Synchronized embryos were placed on the plates and incubated for 72–96 hours at 20°C before scoring. Worms were then scored using Nikon SMZ745 zoom stereomicroscope for PVL, BOW, or Multivulva phenotype. Worms presenting the phenotypes were counted in relative to the total number of worm in each plate. In the RNAi experiments the fraction of worms presenting the phenotype in the empty vector experiment was subtracted from the fraction of worms presenting the phenotype in the unc-22 or lin-1 RNAi experiments.
Ganem N.S., Ben-Asher N., Manning A.C., Deffit S.N., Washburn M.C., Wheeler E.C., Yeo G.W., Ben-Naim Zgayer O., Mantsur E., Hundley H.A, & Lamm A.T. (2019). Disruption in A-to-I Editing Levels Affects C. elegans Development More Than a Complete Lack of Editing. Cell reports, 27(4), 1244-1253.e4.
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