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2 protocols using fructose

1

Aroma Compounds Extraction Protocol

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Yeast extract, sucrose, glucose, and medium with chloramphenicol were obtained from BTL (Łódź, Poland). Galactose was purchased from Acros Organic (Geel, Belgium). Citric acid, Na2HPO4·2H2O, and MgCl2 were obtained from POCH (Gliwice, Poland). Fructose was obtained from Chempur (Piekary Śląskie, Poland). L-Phenylalanine, lactic acid, sodium sulfate, ethyl acetate, dichloromethane, and diethyl ether were purchased from Sigma-Aldrich (Poznań, Poland). Inulin was obtained from Hortimex Plus (Konin, Poland). Spray-dried sour whey and buttermilk were purchased from Laktopol (Suwałki, Poland). The following reference aroma compounds were purchased from Sigma-Aldrich (Poznań, Poland): 2,3-butanedione, acetic acid, 3-methylbutanal, 3-methyl-1-butanol, butanoic acid, isovaleric acid, 3-(methylthio)-propanal, dimethyl trisulfide, phenylacetaldehyde, furaneol, maltol, 2-phenylethanol, ethyl furaneol, (E)-2-nonenal, and phenylacetic acid. The following stable isotopes were obtained from AromaLAB (Freising, Germany): [13C4] 2,3-butanedione, [13C1] acetic acid, [2H3] 2-methylbutanal, [2H2] 3-methyl-1-butanol, [2H7] butanoic acid, [2H2] isovaleric acid, [2H5] 3-(methylthio)-propanal, [2H6] dimethyl trisulfide, [2H5] phenylacetaldehyde, [13C2] furaneol, [2H3] maltol, [2H5] 2-phenylethanol, [2H2] (E)-2-nonenal, and [2H8] naphthalene.
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2

Characterization of Flaxseed Oil Cake

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Flaxseed oil cake (FOC) obtained via cold press technique was kindly donated by ACS Sp. z o.o. (Bydgoszcz, Poland) . The proximate composition of FOC was as follows: solids content 80.50%, protein content 41.97%, carbohydrates content 27.99%, fiber content 6.29%, fat content 6.11%, ash content 4.50%. Lactobacillus rhamnosus GG (ATCC53103), Enterococcus faecalis ATCC29212, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC9027, Salmonella enterica subsp. enterica serovar Typhimurium ATCC14028, Staphylococcus aureus ATCC 43300 (MRSA -Methicillin-resistant Staphylococcus aureus) were purchased from ATCC (Manassas, Virginia, USA). Buffered peptone water, MRS agar and broth, XLD agar, Slanetz and Bartley agar, McConkey agar, Pseudomonas agar base with CN supplement, Mannitol Salt agar were obtained from Merck (Darmstadt, Germany). Phenolphthalein, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Folin-Ciocalteu's reagent, sodium carbonate, gallic acid, sodium nitrite, aluminium chloride, quercetin, and potassium persulphate were purchased from Sigma Aldrich (Darmstadt, Germany). Sodium chloride, sodium peroxide, ethanol, methanol, glucose, and fructose were supplied from Chempur (Piekary Śląskie, Poland). All reagents were of analytical grade.
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