Fos2a icreer trap2 mice

Male C57BL6/J mice were obtained from Australian BioResources. Fos^2A-iCreER/+ (TRAP2) mice were obtained from The Jackson Laboratory (Jax #030323) and crossed with ROSA26-CAG-stop-flox-tdTomato (Ai14) mice (Jax #007914) to produce double transgenic mice that were used in experiments. Transgenic experimental mice were heterozygous for the Fos^2A-iCreER/+ gene and homozygous for the reporter gene.
All mice were 8-12 weeks old at the beginning of experiments. Mice were housed in groups of 2-4 in plastic cages (32 cm × 27 cm × 26 cm) in a temperature-controlled environment (25°C) on a 12 h light-dark cycle (lights on at 0700). All experiments took place during the light phase of the cycle. Food and water were available ad libitum. All procedures were approved by the ethics committee at the Garvan Institute of Medical Research and in accordance with the National Health and Medical Research Council animal experimentation guidelines and the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes (2013).

Animal experiments were approved by The Rockefeller University Animal Care and Use Committee and were carried out in accordance with the National Institutes of Health guidelines. All experiments were conducted on male mice older than 8 weeks of age housed in a 12-h light/12-h dark cycle at 22 °C and 30–60% humidity. We used the following genotypes of mice: C57BL/6J (wild type; number 000664, The Jackson Laboratory), FOS^2A-iCreER (TRAP2) mice (The Jackson Laboratory, stock number 030323), CAG–Sun1/sfGFP (INTACT) mice (The Jackson Laboratory, stock number 021039), VGLUT2–IRES–Cre mice (The Jackson Laboratory, stock no. 028863), VGAT–IRES–Cre mice (The Jackson Laboratory, stock no. 028862), ADCYAP1–2A–Cre mice (The Jackson Laboratory, stock number 030155), PHOX2B–Cre (The Jackson Laboratory, Stock 016223), DBH–Cre (The Jackson Laboratory, stock number 033951), TH–Cre (The Jackson Laboratory, stock number 008601), TAC1–Cre (The Jackson Laboratory, stock number 021877), PDYN–Cre (The Jackson Laboratory, stock number 027958), CCK–Cre (The Jackson Laboratory, stock number 012706), NTS–Cre (The Jackson Laboratory, stock number 017525). For all Cre mouse line experiments, only heterozygous animals were used. Sample sizes were decided based on experiments from similar studies. Animals were randomly assigned to groups when possible.