The following methods reported are in accordance with the ARRIVE guidelines (https://arriveguidelines.org ) for the reporting of animal experiments. The mouse study was performed at Hooke Laboratories, Lawrence, MA, USA. All methods were reviewed and approved by the Hooke and Children’s Mercy IACUC and performed in according to the relevant international laboratory animal welfare guidance and regulations. Mice were sourced from Taconic Biosciences, Rensselaer, NY, USA.
5 BALB/c female mice between 7 and 10 weeks of age were immunized subcutaneously with 0.1 ml/mouse at 37.5 ug/mL of the FKEELDKYFKNHC-CRM197 peptide (Creative Biolabs), mixed 1:1 with Addavax (VAC-ADX-10, Invivogen, San Diego, CA, USA) on days 0, 21, and 35. Serum was collected on day 0, 28, and 42. Baseline sample (day 0) was used as the negative control for each mouse. No randomization or blinding was utilized in this study. Antibody level outcomes were performed across the timepoints during immunization and tested for statistical significance using nonparametric tests due to the exploratory sample size.
5 BALB/c female mice between 7 and 10 weeks of age were immunized subcutaneously with 0.1 ml/mouse at 37.5 ug/mL of the FKEELDKYFKNHC-CRM197 peptide (Creative Biolabs), mixed 1:1 with Addavax (VAC-ADX-10, Invivogen, San Diego, CA, USA) on days 0, 21, and 35. Serum was collected on day 0, 28, and 42. Baseline sample (day 0) was used as the negative control for each mouse. No randomization or blinding was utilized in this study. Antibody level outcomes were performed across the timepoints during immunization and tested for statistical significance using nonparametric tests due to the exploratory sample size.