Micromass quattro premier xe mass spectrometer

Chromatographic separation was carried out on a Finnigan Surveyor HPLC system (Thermo Fisher Scientific, United States) with a Thermo Hypesil Gold C18 (150 mm × 2.1 mm, 5 μm) column at 40°C, which included an online degasser and a Surveyor autosampler. The flow rate was set at 0.25 ml/min. The injection volume was 10 μl. Mobile phase A was methanol, whereas mobile phase B was 0.1% formic acid solvent containing 5 mmol/l ammonium acetate. Using gradient elution, the specific settings are set as follows: 0.0–1.0 min, A/B (10/90); 9.0–15 min, A/B (90/10); and 16.0–22.0 min, A/B (10/95). It is necessary to rinse the needle with H₂O/methanol (50/50, v/v) for 8 min between injections to ensure the accuracy of quantitative results. Mass spectrometric acquisition was performed on a Micromass-Quattro Premier XE mass spectrometer (Waters, Milford MA, United States) using the positive electrospray ionisation mode (ESI+). The capillary voltage and spray voltage were maintained at 3.5 and 4.6 kV, respectively. The sheath and aux gas pressures were maintained at 30 and 10 arb, respectively. The capillary temperature was 350°C, and the tube lens offset was 104 V. The parent ion of SAL was 240.100 m/z. whereas the quantitative daughter ions of SAL were 121.082, 148.060, and 222.163 m/z and the collision energies (Ev) of the daughter ions were 28, 16, and 8 eV.

Duplicate 1 L aqueous phase samples were collected from each of the treatment tanks as well as the system INF and effluent (EF) for quantification of PPCPs by EPA method 1694
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27} at a commercial analytical lab (TestAmerica, Sacramento, CA) using the Waters Acquity UPLC System and Waters Micromass Quattro Premier XE Mass Spectrometer.