Mouse albumin elisa kit

Intestinal permeability was
evaluated by determining the concentration of albumin in feces. Feces
(∼50 mg) were homogenized in 0.5 mL of modified RIPA buffer
using a bead beater, as described in Section 2.3.2. The homogenate was centrifuged (8000g, 5 min, 4 °C), and the concentration of fecal albumin
was determined in the supernatant using the Mouse Albumin ELISA kit
(Cusabio), following the manufacturer’s instructions.
To assess possible signs of endotoxemia, proinflammatory cytokine
IL-1β and lipopolysaccharide-binding protein (LBP) in serum
were assessed using specific ELISA kits [LBP (Cloud-Clone Corporation),
IL-1β (Sigma)].

The cerebral cortex and hippocampus tissues of PAP mice were homogenized in radio immunoprecipitation assay lysis buffer (P0013B, Beyotime, China) containing protease inhibitors (ST506, Beyotime, China), sonicated briefly, lysed on ice for 30 min with gentle agitation, and then centrifuged at 14,800 × g for 20 min at 4°C to remove debris. Aliquots of the lysates were tested for Aβ_1-42 monomers and Aβ oligomers, using the Human/Rat Beta Amyloid (42) ELISA kit (290–62601, Wako, Japan) and Mouse Aβ Oligomer ELISA kit (AB6094, Abmart, China), respectively, according to the manufacturer’s protocols. Plasma and brain tissue IL–1β and TNF–α were determined using the Mouse IL-1β (EMC001b, NeoBioscience, China) and TNF-α (EMC102a, NeoBioscience, China) ELISA kits in accordance with the manufacturer’s protocols. In addition, feces of PAP mice were homogenized at 50 mg/mL in sterilized PBS, and large debris were removed by brief pulsed centrifugation. The fecal suspension was centrifuged at 14,800 × g for 15 min at 4°C to remove sediments, and the levels of fecal LCN2 and albumin were assayed using the Mouse LCN-2/neutrophil gelatinase-associated lipocalin (NGAL) Quantikine ELISA kit (MLCN20, R&D Systems, USA) and Mouse Albumin ELISA kit (E13878m, Cusabio, China).