Near ir viability

Manufactured by Thermo Fisher Scientific

The Near-IR viability is a laboratory instrument that measures the viability of biological samples using near-infrared spectroscopy. It provides a rapid, non-invasive method to assess the overall health and status of cells or tissues.

Automatically generated - may contain errors

Lab products found in correlation

Cellrox deep red flow cytometry assay kit, by Thermo Fisher Scientific (2 mentions) Fortessa flow cytometer, by BD (2 mentions) Glucose glotm assay, by Promega (1 mentions) Almarblue assay, by Thermo Fisher Scientific (1 mentions) Glutamate glotm assay, by Promega (1 mentions) Lactate glotm assay, by Promega (1 mentions)

2 protocols using near ir viability

Latency Activation and ROS Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Activation from latency was measured using GFP (J-Lat 10.6) or HIV-1 core antigen-FITC, KC57 (Beckman Coulter) staining (U1) complemented with Near-IR viability (Invitrogen). Cellular ROS was measured using CellROX^TM Deep red Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s protocol. Experiments were run on Fortessa flow cytometer (BD Bioscience) and analyzed using FlowJo 10.6.2 (TreeStar Inc).

Mikaeloff F., Svensson Akusjärvi S., Ikomey G.M., Krishnan S., Sperk M., Gupta S., Magdaleno G.D., Escós A., Lyonga E., Okomo M.C., Tagne C.T., Babu H., Lorson C.L., Végvári Á., Banerjea A.C., Kele J., Hanna L.E., Singh K., de Magalhães J.P., Benfeitas R, & Neogi U. (2022). Trans cohort metabolic reprogramming towards glutaminolysis in long-term successfully treated HIV-infection. Communications Biology, 5, 27.

+ Open protocol

+ Expand

Evaluating Cytotoxicity and Cellular Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytotoxicity assays: Toxicity of cART regimens (TDF+3TC+EFV and AZT+3TC+EFV) or 2-DG and DON were evaluated using AlmarBlue assay (Invitrogen) over the course of five days, and 48h, respectively, according to the manufacturer's protocol.
Flow cytometry: Activation from latency was measured using GFP (J-Lat 10.6) or HIV-1 core antigen-FITC, KC57 (Beckman Coulter) staining (U1) complemented with Near-IR viability (Invitrogen). Cellular ROS was measured using CellROX TM Deep red Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer's protocol. Experiments were run on Fortessa flow cytometer (BD Bioscience) and analyzed using FlowJo 10.6.2 (TreeStar Inc).
Intracellular metabolite measurement: Intracellular metabolites were measured using Glucose-Glo TM Assay, Lactate-Glo TM Assay, and Glutamate-Glo TM Assay (Promega) according to the manufacturers protocol.

Mikaeloff F., Akusjärvi S.S., Ikomey G.M., Krishnan S., Sperk M., Gupta S., Magdaleno G.D.V., Escós A., Lyonga E., Okomo M.C., Tagne C.T., Babu H., Lorson C.L., Végvári Á., Banerjea A.C., Kele J., Hanna L.E., Singh K., Magalhães J.P.d., Benfeitas R., & Neogi U. (2021). Trans Cohorts Metabolomic Modulation Following Long-Term Successful Therapy in HIV-Infection.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!