Tfe3 hpa023881

Manufactured by Merck Group

Sourced in United States

TFE3 (HPA023881) is a laboratory product manufactured by Merck Group. It is an antibody used for the detection of the TFE3 protein in biological samples.

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Lab products found in correlation

Bca assay, by Thermo Fisher Scientific (2 mentions) Polyvinylidene difluoride membrane, by Merck Group (2 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (2 mentions) Anti raga d8b5, by Cell Signaling Technology (2 mentions) Anti aid maid 2, by Thermo Fisher Scientific (2 mentions) Lamin b 66095 1 ig, by Proteintech (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Phosphatase inhibitor cocktail, by Merck Group (2 mentions) Sds polyacrylamide gel electrophoresis sds page, by Bio-Rad (2 mentions) Anti tfeb a303 673a, by Fortis Life Sciences (2 mentions) Anti raptor 24c12, by Cell Signaling Technology (2 mentions) Tubulin 11224 1 ap, by Proteintech (2 mentions) Tfeb a303 673a, by Fortis Life Sciences (2 mentions) Anti β actin 13e5, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Lamin b1, by Cell Signaling Technology (1 mentions) Phospho s6 s235 236, by Cell Signaling Technology (1 mentions) Nfat1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

HPA023881 (10 citations) Anti-TFE3 (HPA023881) (3 citations) Antibody against TFE3 (HPA023881) (2 citations)

4 protocols using tfe3 hpa023881

Quantitative Western Blot Analysis of B Cell Signaling

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B cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS)) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich). Protein concentration was detected by BCA assay (Thermo Fisher Scientific), and an equal amount of protein was resolved in 4–12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore) and probed overnight with the following primary antibodies: : anti-p-S6K (108D2), anti-p-S6 (D57.2.2E), anti-LAMP1(C54H11), anti-p-4EBP1 (236B4), anti-Raptor (24C12), and anti-RagA (D8B5) all from Cell Signaling Technology), anti-AID (mAID-2, Thermo Fisher Scientific), anti-TFEB (A303–673A, Bethyl Laboratories), Lamin B (66095–1-Ig, Proteintech), tubulin (11224–1-AP, Proteintech), TFE3 (HPA023881, Sigma-Aldrich) and anti-β-actin (13E5, Sigma-Aldrich). The membrane was washed and incubated with indicated secondary antibody for the subsequently enhanced chemiluminescence (ECL, Thermo Fisher) exposure.

Zhu X., Wu Y., Li Y., Zhou X., Watzlawik J.O., Chen Y.M., Raybuck A.L., Billadeau D., Shapiro V., Springer W., Sun J., Boothby M.R, & Zeng H. (2024). Rag-GTPase-TFEB/TFE3 axis controls B cell mitochondrial fitness and humoral immunity independent of mTORC1. Research Square.

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Western Blot Analysis of Signaling Pathways

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B cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS)) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich). Protein concentration was detected by BCA assay (Thermo Fisher Scientific), and an equal amount of protein was resolved in 4–12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore) and probed overnight with the following primary antibodies: anti-p-S6K (108D2), anti-p-S6 (D57.2.2E), anti-LAMP1(C54H11), anti-p-4EBP1 (236B4), anti-Raptor (24C12), and anti-RagA (D8B5) all from Cell Signaling Technology), anti-AID (mAID-2, Thermo Fisher Scientific), anti-TFEB (A303–673A, Bethyl Laboratories), Lamin B (66095–1-Ig, Proteintech), tubulin (11224–1-AP, Proteintech), TFE3 (HPA023881, Sigma-Aldrich) and anti-b-actin (13E5, Sigma-Aldrich). The membrane was washed and incubated with indicated secondary antibody for the subsequently enhanced chemiluminescence (ECL, Thermo Fisher) exposure.

Zhu X., Wu Y., Li Y., Zhou X., Watzlawik J.O., Chen Y.M., Raybuck A.L., Billadeau D., Shapiro V., Springer W., Sun J., Boothby M.R, & Zeng H. (2024). The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness. bioRxiv.

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Immunoblot Analysis of Cellular Proteins

Cited 1 time

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The methods used for the preparation of cell lysates from in vitro and in vivo samples and for immunoblot analyses have been previously described [20 (link)]. The antibodies used included TFE3 (HPA023881, MilliporeSigma, Burlington, MA, USA), ATP6V1A (17115-1-AP, Proteintech, Rosemont, IL, USA), ATP6V1B1 (A-6427, Invitrogen, Waltham, MA, USA), Histone H3 (4499, Cell Signaling Technology, Danvers, MA, USA), LaminB1 (13435, Cell Signaling Technology), pTfeb (Ser142) (AF3845, Affinity Biosciences, Cincinnati, OH, USA), Atg7 (8558, Cell Signaling Technology), GAPDH (2118S, Cell Signaling Technology), αTubulin (T6199, Sigma-Aldrich, Burlington, MA, USA) and Tfeb (A303-673A, Bethyl Laboratories, Montgomery, TX, USA; sc-48784, Santa Cruz Biotechnology, Dallas, TX, USA).

Nah J., Sung E.A., Zhai P., Zablocki D, & Sadoshima J. (2022). Tfeb-Mediated Transcriptional Regulation of Autophagy Induces Autosis during Ischemia/Reperfusion in the Heart. Cells, 11(2), 258.

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Antibody Panel for Autophagy Signaling

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Antibodies used in this study were as follows: ATG16L1 (8089, human), phospho-ATG14 S29 (92340), ATG14 (96752), phospho-Beclin S30 (54101), FIP200 (12436), FLCN (3697), GABARAPL1 (26632), GABARAPL2 (14256), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [5174, 1:10,000 for WB (Western blot)], DYKDDDDK tag (14793), HA tag (3724), myc tag (2278), LC3A/B (12741), LC3B (3868), LAMTOR1 (8975), LAMP1 [15665, 1:1000 for immunofluorescence (IF)], NFAT1 (5861, 1:250 for IF), NPRL2 (37344), phospho-S6K (9234), S6K (2708), phospho-S6 S235/236 (4858, 1:3000 for WB), S6 (2217, 1:5000 for WB), TAX1BP1 (5105), TFEB (4240), TFEB (37785, 1:200 for IF), and phospho-ULK S757 (14202) were from Cell Signaling Technology. Mouse monoclonal anti–S. Typhimurium lipopolysaccharide (clone 1E6, ab8274) and FNIP1 (ab134969) were from Abcam. TFE3 (HPA023881) was from MilliporeSigma. p62 (GP62-C) was from Progen. Galectin-3 (sc-23938) was from Santa Cruz Biotechnology. TFEB (A303-673A, 1:200 for IF in murine cells) was from Bethyl Laboratories. All antibodies were used at a 1:1000 dilution for Western blotting unless otherwise noted.

Goodwin J.M., Walkup WG I.V., Hooper K., Li T., Kishi-Itakura C., Ng A., Lehmberg T., Jha A., Kommineni S., Fletcher K., Garcia-Fortanet J., Fan Y., Tang Q., Wei M., Agrawal A., Budhe S.R., Rouduri S.R., Baird D., Saunders J., Kiselar J., Chance M.R., Ballabio A., Appleton B.A., Brumell J.H., Florey O, & Murphy L.O. (2021). GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis. Science Advances, 7(40), eabj2485.

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