Oligonucleotides for real time polymerase chain reaction

Cell culture media were purchased from Invitrogen (Thermo Fisher Scientific MA, USA). Fetal bovine serum (FBS) was from Hyclone Laboratories Inc. (Logan, UT, USA). Calcitriol (1α,25-dihidroxivitamina D₃) was purchased from Sigma (St. Louis, MO, USA), seocalcitol (EB1089) was obtained from Tocris Bioscience (Bristol, United Kingdom), and IL-1β was purchased form R&D Systems (Minneapolis, USA). TNF-α was obtained from PeproTech (USA); TRIzol and the oligonucleotides for real-time polymerase chain reaction (qPCR) were from Invitrogen. The TaqMan Master reaction, probes, plates, and reverse transcription (RT) system were all purchased from Roche Diagnostics (Mannheim, Germany).

Estradiol (E₂), 4-hydroxytamoxifen and calcipotriol (MC 903) were purchased from Sigma (St. Louis, MO, USA). Cell culture medium was obtained from Life Technologies (Grand Island, NY, USA). Fetal bovine serum (FBS) was from Hyclone Laboratories Inc. (Logan, UT, USA) and the antiestrogen ICI-182,780 (Fulvestrant) from Zeneca Pharmaceuticals (Wilmington, DE, USA). Gefitinib (Iressa, ZD1839) was kindly provided by AstraZeneca (Wilmington, DE, USA). U0126 was from Millipore (MA, USA). Trizol and the oligonucleotides for real time polymerase chain reaction (qPCR) were from Invitrogen (CA, USA). The TaqMan Master reaction, probes, capillaries, reverse transcription (RT) system and the cell proliferation assay (XTT) were purchased from Roche (Roche Applied Science, IN, USA). MCF-7 nuclear extract was purchased from Santa Cruz Biotechnology Inc., (CA, USA). The VDR antagonist (23S)-25-dehydro-1-hydroxyvitamin D3-26,23-lactone (TEI-9647) and 1α,25-dihydroxycholecalciferol (calcitriol) were kindly donated from Teijin Pharma Limited (Tokyo, Japan) and Hoffmann-La Roche Ltd. (Basel, Switzerland), respectively.