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Qpcr supermix low rox pcr assay

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The QPCR SuperMix Low Rox PCR assay is a reagent designed for quantitative real-time PCR (qPCR) applications. It contains all the necessary components for performing qPCR reactions, including a DNA polymerase, dNTPs, and a low concentration of the passive reference dye ROX.

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Lab products found in correlation

Qiaamp dsp dna blood mini kit, by Qiagen (2 mentions) Dna prep kit, by Illumina (2 mentions) 7500 fast dx real time pcr instrument, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PCR SuperMix (56 citations) QPCR assays (4 citations)

2 protocols using qpcr supermix low rox pcr assay

1

Orthopoxvirus Detection from Lesion Swabs

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A total of 9 lesion swab clinical specimens were tested. DNA was extracted from clinical specimens using the QIAGEN QIAamp DSP DNA Blood Mini kit and tested with Perfecta Multiplex qPCR SuperMix Low Rox PCR assay in conjunction with the CDC FDA-approved Non-variola Orthopoxvirus (VAC1) assay on the Applied Biosystems 7500 Fast Dx Real-Time PCR instrument. Libraries were prepared for sequencing using the Illumina DNA prep kit. Pooled libraries were sequenced on the Illumina MiSeq, with 0.8 to 1.2 million reads per library.
Chen N.F., Chaguza C., Gagne L., Doucette M., Smole S., Buzby E., Hall J., Ash S., Harrington R., Cofsky S., Clancy S., Kapsak C.J., Sevinsky J., Libuit K., Park D.J., Hemarajata P., Garrigues J.M., Green N.M., Sierra-Patev S., Carpenter-Azevedo K., Huard R.C., Pearson C., Incekara K., Nishimura C., Huang J.P., Gagnon E., Reever E., Razeq J., Muyombwe A., Borges V., Ferreira R., Sobral D., Duarte S., Santos D., Vieira L., Gomes J.P., Aquino C., Savino I.M., Felton K., Bajwa M., Hayward N., Miller H., Naumann A., Allman R., Greer N., Fall A., Mostafa H.H., McHugh M.P., Maloney D.M., Dewar R., Kenicer J., Parker A., Mathers K., Wild J., Cotton S., Templeton K.E., Churchwell G., Lee P.A., Pedrosa M., McGruder B., Schmedes S., Plumb M.R., Wang X., Barcellos R.B., Godinho F.M., Salvato R.S., Ceniseros A., Breban M.I., Grubaugh N.D., Gallagher G.R, & Vogels C.B. (2023). Development of an amplicon-based sequencing approach in response to the global emergence of mpox. PLOS Biology, 21(6), e3002151.
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2

Rapid Orthopoxvirus Detection from Clinical Samples

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A total of 9 lesion swab clinical specimens were tested. DNA was extracted from clinical specimens using the QIAGEN QIAamp DSP DNA Blood Mini kit and tested with Perfecta Multiplex qPCR SuperMix Low Rox PCR assay in conjunction with the CDC FDA-approved Non-variola Orthopoxvirus (VAC1) assay on the Applied Biosystems® 7500 Fast Dx Real-Time PCR instrument. Libraries were prepared for sequencing using the Illumina DNA prep kit. Pooled libraries were sequenced on the Illumina MiSeq, with 0.8–1.2 million reads per library.
Chen N.F., Chaguza C., Gagne L., Doucette M., Smole S., Buzby E., Hall J., Ash S., Harrington R., Cofsky S., Clancy S., Kapsak C.J., Sevinsky J., Libuit K., Park D.J., Hemarajata P., Garrigues J.M., Green N.M., Sierra-Patev S., Carpenter-Azevedo K., Huard R.C., Pearson C., Incekara K., Nishimura C., Huang J.P., Gagnon E., Reever E., Razeq J., Muyombwe A., Borges V., Ferreira R., Sobral D., Duarte S., Santos D., Vieira L., Gomes J.P., Aquino C., Savino I.M., Felton K., Bajwa M., Hayward N., Miller H., Naumann A., Allman R., Greer N., Fall A., Mostafa H.H., McHugh M.P., Maloney D.M., Dewar R., Kenicer J., Parker A., Mathers K., Wild J., Cotton S., Templeton K.E., Churchwell G., Lee P.A., Pedrosa M., McGruder B., Schmedes S., Plumb M.R., Wang X., Barcellos R.B., Godinho F.M., Salvato R.S., Ceniseros A., Breban M.I., Grubaugh N.D., Gallagher G.R, & Vogels C.B. (2023). Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus. medRxiv.
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