The thermostability of 146S in solution and encapsulated in ZIF-8 was measured by differential scanning fluorescence (DSF) analysis according to our published method.41 (link) Briefly, 18 μL solution containing 146S was mixed with 2 μL 1 : 100-fold diluted SYBR Green II (Solarbio, China). The experiments were performed using a real-time PCR instrument (Applied Biosystem, USA) at a scan rate of 1 °C min−1 from 25 °C to 95 °C. The Tm value corresponding to the dissociation of 146S to 12S was calculated according to the maximum of the first derivative [d(RFU)/dT] plot of the fluorescence curve.
Sybr green 2
Manufactured by Solarbio
Sourced in China
SYBR Green II is a fluorescent dye used for nucleic acid detection and quantification. It binds to double-stranded DNA, emitting a green fluorescent signal upon excitation. The dye can be used in various applications, such as real-time PCR, gel electrophoresis, and DNA-binding assays.
Automatically generated - may contain errors
Lab products found in correlation
Real time pcr instrument, by Thermo Fisher Scientific (1 mentions)
Aptamer, by Sangon (1 mentions)
Direct q water purification system, by Merck Group (1 mentions)
Protein free blocking buffer, by Thermo Fisher Scientific (1 mentions)
β glucuronidase, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
2 protocols using sybr green 2
1
Thermostability of 146S Virus Capsids
2
Aptamer-Based Gold Nanoparticle Assay
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Hydrogen tetrachloroaurate(iii ) trihydrate (HAuCl4·3H2O), thrombin and aptamers were purchased from Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China). Methanol (HPLC grade) was purchased from Merck (Billerica, MA, USA). Nitrofurantoin (the internal standard, IS) was provided by Nine-Dinn Chemistry Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA), Heparin and β-glucuronidase were supplied by Sigma-Aldrich (St. Louis., MO, USA). SYBR Green II was obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Protein-free blocking buffer was purchased from Thermo Fisher Scientific (Madison, USA). The purified water was prepared by Direct-Q water purification system (Millipore, USA). The amino-modified magnetic beads (MBs, diameter 1–2 μm) were purchased from Baseline Chromatographic Technique Centre (Tianjin, China). The sequences of aptamers as follows,
Aptamer 29: 5′-NH2-T12-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′;
Aptamer 15: 5′-SH-T5-GGT TGG TGT GGT TGG-3′.
Aptamer 29: 5′-NH2-T12-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′;
Aptamer 15: 5′-SH-T5-GGT TGG TGT GGT TGG-3′.
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