Ac 400 mhz

All chemical reagents and solvents were obtained from Sigma-Aldrich (Ireland) Ltd. and unless otherwise stated were used without further purification. C8-Alkyne-dU-CEP was purchased from BaseClick GmbH. NMR spectra were recorded on Bruker AC 400 MHz or 600 MHz spectrometers (Supplementary S1). FT-IR spectra were collected on Perkin Elmer Spectrum Two spectrometer. ESI-MS analysis was performed on a Bruker HCT Mass Spectrometer. pH was monitored using a Mettler Toledo InLab Expert Pro-ISM pH probe. Crystallographic data was collected at 100(1)K on a Synergy Dualflex, AtlasS2 diffractometer (Supplementary S2). The structure was solved by dual space methods and refined on F2 using all the reflections (SHELXL-2018). Mass analysis of oligonucleotides was characterised at Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) on a Bruker Daltonics Autoflex II instrument (Supplementary S3). Thermal melting experiments were conducted on Agilent Cary 100 UV-Vis dual beam spectrophotometer equipped with a 6 × 6 Peltier multicell system with temperature controller. DNA was quantified on a Jasco UV-Vis spectrophotometer. Polyacrylamide gels were imaged on Syngene G:Box mini 9 gel documentation system.