Adhesivecap 200 opaque

Manufactured by Zeiss

Sourced in Germany

The AdhesiveCap 200 Opaque is a laboratory equipment product designed for sealing sample containers. It features an opaque cap that provides a secure, airtight closure to protect the contents of the container.

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Lab products found in correlation

Membraneslide 1.0 pen, by Zeiss (1 mentions) Palm microbeam, by Zeiss (1 mentions) Cm3050s cryostat, by Leica (1 mentions) Repli g ffpe kit, by Qiagen (1 mentions) Repli g mini kit, by Qiagen (1 mentions) Pen membrane slide, by Zeiss (1 mentions) Cm3050, by Leica (1 mentions) Microbeam 4, by Zeiss (1 mentions) Lcm staining kit, by Thermo Fisher Scientific (1 mentions) Rneasy micro kit, by Qiagen (1 mentions) Palm microbeam system, by Zeiss (1 mentions) Mayer s hemalum solution, by Merck Group (1 mentions) Picopure rna isolation kit, by Thermo Fisher Scientific (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions)

Close spelling variants of this product

AdhesiveCap (14 citations) Opaque AdhesiveCaps (2 citations) AdhesiveCap 200 opaque cover (2 citations)

4 protocols using adhesivecap 200 opaque

Laser-Capture Microdissection of Brain Neurons

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Flash-frozen brain tissue from 5-mo-old WT and Tg rats was cut into 10-μm-thick sections using a Leica CM3050S cryostat. Tissue was thaw-mounted onto 1.0-mm PEN membrane-covered glass slides (MembraneSlide 1.0 PEN; Carl Zeiss), which were irradiated with UV light for 30 min prior to cryosectioning. Mounted tissue sections were stained with Cresyl violet, as described in SI Appendix.
The entire pyramidal layer of the subiculum and CA1 was microdissected from 40 tissue sections per animal using the PALM MicroBeam (Carl Zeiss). Neurons were identified based on their characteristic Cresyl violet staining: Neurons stained diffusely throughout the entire cell body, while glial cells exhibited darker staining exclusively within the nucleus. Microdissected neurons were pressure-catapulted into 200-μL PCR tubes with opaque adhesive cap (AdhesiveCap 200 Opaque; Carl Zeiss) using the following UV-laser settings: Cut energy, 75; cut focus, 70; auto-LPC dot-size, 12 (SI Appendix, Fig. S1A). mRNA extraction was performed immediately following LCM, as detailed in SI Appendix.

Welikovitch L.A., Do Carmo S., Maglóczky Z., Malcolm J.C., Lőke J., Klein W.L., Freund T, & Cuello A.C. (2020). Early intraneuronal amyloid triggers neuron-derived inflammatory signaling in APP transgenic rats and human brain. Proceedings of the National Academy of Sciences of the United States of America, 117(12), 6844-6854.

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Extracting DNA from FFPE Tissue

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Serial unstained FFPE tissue sections (10 μm) were aligned with the corresponding H&E stained section in which the location of the FTE was pen marked. With a sterile, single-use scalpel blade tip the FTE tissue was scrapped off the glass slide and mounted onto 200 μl a membrane cap (AdhesiveCap 200 opaque, Zeiss, Jena, Germany). To avoid cross contamination, the slides and caps were placed in sterile tissue culture dishes. DNA extraction and amplification was performed using the REPLIg FFPE kit (Qiagen). For macrodissection of the p53 signature from FFPE immuno-stained sections the slides were incubated overnight in Xylene at 37 °C. The coverslip was lifted and the slide soaked in Xylene for 30 min, then washed in gradient ethanol. The tissue was macrodissected using a scalpel blade tip. DNA was extracted with the Arcturus® PicoPure® DNA isolation kit (Life technologies) and amplified using the REPLIg mini kit (Qiagen) according to manufacturer's instructions. DNA was quantified as described below.

Hellner K., Miranda F., Fotso Chedom D., Herrero-Gonzalez S., Hayden D.M., Tearle R., Artibani M., KaramiNejadRanjbar M., Williams R., Gaitskell K., Elorbany S., Xu R., Laios A., Buiga P., Ahmed K., Dhar S., Zhang R.Y., Campo L., Myers K.A., Lozano M., Ruiz-Miró M., Gatius S., Mota A., Moreno-Bueno G., Matias-Guiu X., Benítez J., Witty L., McVean G., Leedham S., Tomlinson I., Drmanac R., Cazier J.B., Klein R., Dunne K., Bast RC J.r., Kennedy S.H., Hassan B., Lise S., Garcia M.J., Peters B.A., Yau C., Sauka-Spengler T, & Ahmed A.A. (2016). Premalignant SOX2 overexpression in the fallopian tubes of ovarian cancer patients: Discovery and validation studies. EBioMedicine, 10, 137-149.

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Laser Microdissection and RNA Extraction from Keloid and Normal Skin

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Serial 8μm cryosections (Leica CM3050S, UK) of OCT-embedded keloid and NS samples were cut onto specialised polyethylene naphthalate (PEN) membrane slides (Carl Zeiss, UK). To differentiate epidermis from dermis, whilst preserving tissue RNA integrity, a rapid staining protocol was performed (LCM Staining Kit, Ambion, Austin TX, USA) according to the manufacturer’s instructions [16 (link), 17 (link)]. Using a P.A.L.M. LCM microscope (Carl Zeiss MicroBeam 4.2, Germany) epidermis and dermis of each sample was laser cut and catapulted away from the slide into separate overhanging microtube caps (AdhesiveCap 200 Opaque, Carl Zeiss Microscopy Ltd, Cambridge, UK). Multiple ‘elements’ were captured from each tissue section of least three sequential sections from each patient, ensuring adequate biological representation. The captured tissue was mixed with lysis buffer (Buffer RLT with 1% 2-mercaptoethanol, RNeasy Micro Kit, Qiagen, UK) and stored at -80°C until extraction according to manufacturer’s instructions (RNeasy Micro Kit, Qiagen, UK). Following extraction, the samples were again stored at -80°C [18 (link)].

Jumper N., Hodgkinson T., Paus R, & Bayat A. (2017). Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology. PLoS ONE, 12(3), e0172955.

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Laser Capture Microdissection and Gene Expression Analysis

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Briefly, sagittal mouse brain cryostat sections (12 mm) were placed on UV light pre-treated PEN membrane slides, washed in PBS for 5 min, stained with Mayer's hemalum solution (Merck, Darmstadt, Germany) and dehydrated in ascending ethanol series and stored at À80 C overnight. Laser capture microdissection was performed using a PALM MicroBeam system (Zeiss, Oberkochen, Germany). Cells were collected in PCR tubes (AdhesiveCap 200 opaque, Zeiss, Oberkochen, Germany) and stored for a short time at À80 C (up to 1 week). RNA isolation was performed using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA) with DNase treatment. cDNA synthesis was carried out with iScript cDNA Synthesis Kit (Bio-Rad) with subsequent PCR using the following primers: Venus_For: 5 0 -GAC GAC GGC AAC TAC AAG AC-3 0 , Venus_Rev: 5 0 -CCA CCA TCG ATC TGC TTG TC-3 0 ; CaMKIIa_For: 5 0 -CAG CAT CCC AGC CCT AGT TC-3 0 , CaMKIIa_Rev: 5 0 -CCC CAC CAG TAA CCA GAT CG-3 0 ; GAPDH_For: 5 0 -AGT ATG ACT CCA CTC ACG GCA A-3 0 , GAPDH_Rev: 5 0 -ATA CTC AGC ACC GGC CTC ACC-3 0 . PCR was verified by agarose gel electrophoresis.

Kiechle M., von Einem B., Höfs L., Voehringer P., Grozdanov V., Markx D., Parlato R., Wiesner D., Mayer B., Sakk O., Baumann B., Lukassen S., Liss B., Ekici A.B., Ludolph A.C., Walther P., Ferger B., McLean P.J., Falkenburger B.H., Weishaupt J.H, & Danzer K.M. (2019). In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8-16-mer Oligomer Species. Cell reports, 29(9).

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