Plasma levels of hG-CSF were analyzed using the hG-CSF Quantikine enzyme-linked immunosorbent assay (ELISA) kit (SCS50; R&D Systems). Serum rituximab levels were analyzed using the rituximab ELISA kit (IG-AB106; Eagle Biosciences). For anti–hG-CSF, 5J8, and anti–IL-5 mAb ELISAs, immunoassay plates were coated with antigen (BioLegend 578606 and Sino Biologicals 11085-V08H and 15673-HNCE, respectively) and blocked with 3% bovine serum albumin. After binding of expressed serum proteins, detection was carried out with enzyme-linked anti-human IgG and 3,3′,5,5′-tetramethylbenzidine substrate (AP113P; Millipore). For the anti-rituximab ELISA, the coat protein was rituximab from InvivoGen, and the detection antibody was goat anti-mouse IgG H+L horseradish peroxidase from Thermo Fisher.
Goat anti mouse igg h l horseradish peroxidase
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Goat anti-mouse IgG H+L horseradish peroxidase is a secondary antibody conjugate used in immunoassays and other applications where detection of mouse immunoglobulins is required. The antibody is specific for the heavy and light chains of mouse IgG and is coupled to the enzyme horseradish peroxidase, which can be used to detect and quantify the presence of the target antigen.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ap113p, by Merck Group (1 mentions)
Rituximab, by InvivoGen (1 mentions)
Np0322box, by Thermo Fisher Scientific (1 mentions)
Ib401002, by Thermo Fisher Scientific (1 mentions)
Np0002, by Thermo Fisher Scientific (1 mentions)
Supersignal west femto substrate, by Thermo Fisher Scientific (1 mentions)
2 protocols using goat anti mouse igg h l horseradish peroxidase
1
Quantifying Serum Levels of Biotherapeutics
2
Quantitative Western Blotting Assay
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The total protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific Europe bv) following the manufacturer’s instructions. Western blotting was performed using a 4–12% Bis-Tris protein gel, with MES SDS running buffer and transfer onto a polyvinylidene difluoride membrane (respectively NP0322Box, NP0002, and IB4010-02, Thermo Fisher Scientific Europe bv). For Western blotting, a 10 µg/sample was used for lysates and a fixed amount of supernatant. Proteins were visualized using antibodies against Core (Dako, Glostrup, Denmark) and Pol (Santa Cruz Biotechnology, Dallas, TX, USA); goat anti-rabbit IgG horseradish peroxidase (GE Healthcare Life Sciences, Hoegaarden, Belgium) and goat anti-mouse IgG (H + L) horseradish peroxidase (Thermo Fisher Scientific Europe bv) secondary detection systems were employed. Read out was performed using SuperSignal West Femto substrate (Thermo Fisher Scientific Europe bv).
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