Ht 7700 exalens instrument

Contact angle measurement was carried out using SmartDrop software with a UNI-CAM (GITSOFTTECH) system. The surface tension was measured using KRÜSS BP100. The viscosity was measured using an Ubbelohde viscometer (Fungilab). We measured the thickness of the PQD films with the Dektak XT profiler. The AFM measurements were performed by Asylum Research MFP-3D-SA, operating under the tapping mode. The SEM images were collected in a GeminiSEM 500 with an accelerating voltage of 3 kV. Samples for transmission electron microscope (TEM) imaging were taken on a Hitachi HT7700 Exalens instrument with a 300 kV acceleration voltage. The PLQY of the PQD films was recorded using a Horiba Fluorolog-3 system with a Petite Integrating Sphere at an excitation wavelength of 405 nm. The TRPL was recorded using a Horiba time-correlated single-photon counting system at an excitation wavelength of 369 nm.

Liposomes with concentration of 5 μM were visualized using transmission electron microscopy on a Hitachi HT 7700 Exalens instrument (Hitachi, Tokyo, Japan) at an accelerating voltage of 100 kV. Sample was deposited on copper grid (Ted Pella, Pella, IA, USA) with a carbon-formvar support film and dried at room temperature within 2 h. Diameter of liposomes was then analyzed using ImageJ software (version number 1.53t).

Lipid nanoparticles were visualized using TEM on a Hitachi HT 7700 Exalens instrument (Hitachi, Tokyo, Japan). Nanoparticle dispersion for imaging was diluted to 5 μM and deposited on a copper grid (Ted Pella, Pella, IA, USA) with a 15–25 nm carbon-formvar support film. Then, the sample was dried at room temperature for 60 min. The analysis was carried out at an accelerating voltage of 100 kV.