Sodium acetate (140 g, 99%, anhydrous; Sigma-Aldrich) was dissolved in 100 ml of deionized water at 65°C to get a saturated solution. The Sodium acetate solution cooled down to room temperature, and it formed a supersaturated solution for phase transition. The PDMS (10:1 ratio of the base and curing agent; SYLGARD 184, Dow Corning) chambers were designed for various thicknesses by different glass blocks. The PDMS chamber and the transparent microheater were glued together with PDMS adhesive (3140 RTV Coating, Dow Corning) for solution sealing. Then, the Sodium acetate solution was discreetly injected into the PDMS chamber.
3140 rtv coating
Manufactured by Dow
3140 RTV Coating is a one-part, room temperature vulcanizing (RTV) silicone coating that cures to a flexible, durable, and transparent film. It is designed to provide protection and insulation for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 3140 rtv coating
1
Phase Transition Sodium Acetate Protocol
2
Fabrication of Tetrode Microelectrodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 40 cm of tungsten wire (12.5 μm, HFV insulation, California Fine Wire Company) were twisted into a tetrode using an Arduino based twister (Open Ephys). The twisted wires were heat fused by a heat gun (temperature: 220 °C) from three sides. Eight of these tetrodes were attached to a custom-made electrode interface board (EIB, design files in the repository) with gold pins (Neuralynx). A thin layer of silicone was used as a conformal coating (3140 RTV Coating, Dow Corning Inc.). Following assembly, the electrode tips of the assembled microdrive were lowered into a cyanide-free gold plating solution (31 g/l, SIFICO ASC) and plated to 150 kOhm (plating current: -0.015 µA, 1 s, 30 runs, NanoZ, Multi Channel Systems).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!