Icelandic seaweeds U. lactuca (green algae), A. esculenta (brown algae) and P. palmata (red algae) were provided by Icelandic Blue Mussel and Seaweed, which harvested seaweeds in Breidafjordur (West-Iceland). After harvesting the seaweeds were dried (to approximately 90% dry material), milled and delivered vacuum packed. Samples were kept in a dry and dark place at room temperature until used.
Tyrosinase from mushroom, L-3,4-dihydroxyphenylalanine (L-DOPA), elastase from porcine pancreas, ascorbic acid, N-Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), hyaluronidase from bovine testes, quercetin, α-tocopherol, tannic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tripyridyl-s-Triazine (TPTZ), Trolox, Folin-Ciocalteu reagent, gallic acid and a collagenase activity colorimetric assay kit (MAK293) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Hyaluronic acid sodium salt was purchased from MakingCosmetics (Redmond, WA, USA). All other chemicals and reagents used were analytical grade and obtained from VWR International, LLC. Deionized water (Elix® Essential, Merck, Darmstadt, Germany) was used for the extraction and preparation of water-based solutions.
Tyrosinase from mushroom, L-3,4-dihydroxyphenylalanine (L-DOPA), elastase from porcine pancreas, ascorbic acid, N-Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), hyaluronidase from bovine testes, quercetin, α-tocopherol, tannic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tripyridyl-s-Triazine (TPTZ), Trolox, Folin-Ciocalteu reagent, gallic acid and a collagenase activity colorimetric assay kit (MAK293) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Hyaluronic acid sodium salt was purchased from MakingCosmetics (Redmond, WA, USA). All other chemicals and reagents used were analytical grade and obtained from VWR International, LLC. Deionized water (Elix® Essential, Merck, Darmstadt, Germany) was used for the extraction and preparation of water-based solutions.