Various molecular biology reagents

Various molecular biology reagents were purchased from Invitrogen Life Technologies (Gaithersburg, MD); Stratagene(La Jolla), CA; and Promega Biotech (Madison, WI). All DNA and protein size markers were purchased from Invitrogen Life Technologies (Gaithersburg, MD). The mammalian expression vector was purchased from Clontech (Palo Alto, CA). Various antibodies were obtained from Cell Signaling Technology (Boston, MA); Roche Molecular Biochemicals (Indianapolis, IN); Santa Cruz Biotechnology(Dallas, TX); Abcam Inc(Cambridge, MA) and Abclonal Inc (Wuhan, China). The culture medium and most other chemicals and antibiotics were purchased from Sigma (St. Louis, MO) and Invitrogen Life Technologies(Gaithersburg, MD).

Various molecular biology reagents were purchased from Invitrogen Life Technologies, Gaithersburg, MD; Stratagene, La Jolla, CA and Promega Biotech, Madison, WI. All the oligos, DNA and protein size markers were purchased from Invitrogen Life Technologies, Gaithersburg, MD and Sangon Biotech (Shanghai) Co., Ltd. Various antibodies were obtained from Cell Signaling Technology, Boston, MA; abCam Inc., Cambridge, MA; Santa Cruz Biotechnology, Inc. Dallas, TX; Sigma-Aldrich, St. Louis, MO; Transduction Laboratories, San Diego, CA. The culture medium, and most other chemicals and antibiotics were purchased from Sigma-Aldrich, St. Louis, MO and Invitrogen Life Technologies, Gaithersburg, MD.

Various molecular biology reagents were purchased from Invitrogen Life Technologies (Gaithersburg, MD, USA). All the oligos were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Protein size markers were purchased from GenStar Co., Ltd. (Beijing, China). Various antibodies were obtained from Cell Signaling Technology (Boston, MA, USA); Abcam Inc. (Cambridge, MA, USA); Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); Sigma-Aldrich (St. Louis, MO, USA); Proteintech Co., Ltd. (Wuhan, China); Ray Biotech Co., Ltd. (Beijing, China).