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Reference compounds of theobromine

Manufactured by Merck Group
Sourced in Belgium

Reference compounds of theobromine are used as standards for the identification and quantification of theobromine in analytical procedures. Theobromine is a naturally occurring alkaloid found in cocoa, tea, and other plant sources. These reference compounds provide a reliable and consistent baseline for analytical testing.

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2 protocols using reference compounds of theobromine

1

Cocoa Powder Characterization Protocol

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Commercial fat-reduced cocoa powder (10-12% w/w) was supplied by Cargill (Schiphol, Netherlands). Analytical grade NaOH (98%) for alkalization was purchased from Chem-Lab NV (Zedelgem, Belgium) and demineralized water was purified using Elix type 2 pure water system 20 from Merck Millipore (Overijse, Belgium). Methanol (≥99.8%) and formic acid (98%) were obtained from Fischer Scientific (Loughborough, UK), while n-hexane (≥97%), acetone (99.8%) and acetic acid (99.8%) were purchased from Acros Organics (Geel, Belgium) . Reference compounds of theobromine, caffeine, epicatechin and catechin (>98% purity) were provided by Sigma Aldrich (Overijse, Belgium). All the other chemicals were of highest grade commercially available.
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2

Cocoa Powder Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial fat-reduced cocoa powder (10-12% w/w) was supplied by Cargill (Schiphol, Netherlands). Analytical grade NaOH (98%) for alkalization was purchased from Chem-Lab NV (Zedelgem, Belgium) and demineralized water was purified using Elix type 2 pure water system 20 from Merck Millipore (Overijse, Belgium). Methanol (≥99.8%) and formic acid (98%) were obtained from Fischer Scientific (Loughborough, UK), while n-hexane (≥97%), acetone (99.8%) and acetic acid (99.8%) were purchased from Acros Organics (Geel, Belgium) . Reference compounds of theobromine, caffeine, epicatechin and catechin (>98% purity) were provided by Sigma Aldrich (Overijse, Belgium). All the other chemicals were of highest grade commercially available.
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