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Endothelial growth medium 2 bullet kit egm 2

Manufactured by Lonza
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Sourced in United States, Switzerland

Endothelial Growth Medium-2 Bullet Kit (EGM™-2) is a cell culture medium designed to support the growth and proliferation of endothelial cells. It contains a specially formulated combination of supplements and growth factors necessary for the optimal performance of endothelial cell cultures.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Calcein am solution, by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Huvec pcs 100 010, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Amphotericin b, by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) 1640 medium, by Thermo Fisher Scientific (1 mentions) Quercetin hydrate, by Merck Group (1 mentions) Endothelial basal medium 2 ebm 2, by Lonza (1 mentions) Hplc grade methanol, by Merck Group (1 mentions) Iron 3 chloride, by Merck Group (1 mentions)

Close spelling variants of this product

Endothelial Cell Growth Medium-2 (EGM-2TM) supplemental bullet kit Endothelial bullet kit Endothelial growth medium EGM-2 medium EGM-2 kit EGM-2

3 protocols using endothelial growth medium 2 bullet kit egm 2

1

Mussel-Derived Endothelial Cell Assays

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Blue mussel (Mytilus edulis) was purchased from Yeosu Fisheries Co. (Yeosu, Korea). Calcein AM solution, propidium iodide (PI), 2′7′-dichlorofluorescein diacetate (DCFH-DA), H2O2, Hoechst 33342, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Trypsin-EDTA solution and PBS were purchased from Hyclone (Logan, UT, USA). The HUVEC (PCS-100-010™) are produced by the American Type Culture Collection (Rockville, MD, USA). Endothelial Growth Medium-2 Bullet Kit (EGM™-2) was produced by Lonza, (Walkersville, MD, USA).
Suryaningtyas I.T., Ahn C.B, & Je J.Y. (2021). Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury. Marine Drugs, 19(11), 609.
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2

Culturing Lymphatic and Blood Endothelial Cells

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Primary (adult) human dermal lymphatic microvascular endothelial cells (HMVEC-dLyAd; LECs) and human dermal microvascular endothelial cells (HMVEC-dAd; BECs) were purchased from Lonza and passaged in an Endothelial Growth Medium-2 Bullet kit (EGM-2) (Lonza). THP-1 monocytes (ECACC, Public Health England) were passaged in RPMI 1640 (MilliporeSigma) supplemented with 10% FBS (MilliporeSigma), 100 IU/mL penicillin/streptomycin (MilliporeSigma), and 2.5 mg/L amphotericin B (MilliporeSigma). All cells were maintained at 37°C and 5% CO2.
Furlong-Silva J., Cross S.D., Marriott A.E., Pionnier N., Archer J., Steven A., Merker S.S., Mack M., Hong Y.K., Taylor M.J, & Turner J.D. (2021). Tetracyclines improve experimental lymphatic filariasis pathology by disrupting interleukin-4 receptor–mediated lymphangiogenesis. The Journal of Clinical Investigation, 131(5), e140853.
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3

Quercetin Hydrate HPLC Analysis

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Quercetin hydrate, HPLC-grade methanol, and iron (III) chloride were purchased from Sigma-Aldrich (MO, USA). Potassium hexacyanoferate (II) trihydrate was purchased from Merck (Darmstadt, Germany). Roswell Park Memorial Institute (RPMI) 1640 medium and fetal bovine serum (FBS) were obtained from Thermo Fisher Scientific (MA, USA). Endothelial Growth Medium-2 Bullet Kit (EGM-2) and Endothelial Basal Medium-2 (EBM-2) were purchased from Lonza (Basel, Switzerland). All chemicals were of analytical grade. Ultrapure water (specific resistivity of 18.2 MΩ·cm at 25°C) was prepared using a PURELAB Option-Q system (ELGA LabWater; High Wycombe, UK).
Papan P., Kantapan J., Sangthong P., Meepowpan P, & Dechsupa N. (2020). Iron (III)-Quercetin Complex: Synthesis, Physicochemical Characterization, and MRI Cell Tracking toward Potential Applications in Regenerative Medicine. Contrast Media & Molecular Imaging, 2020, 8877862.
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