Nexus 6700 spectrometer

Polymer functionalization was evaluated through NMR and FT-IR analyses. 1 H-NMR spectra were carried out on a Bruker AC (400 MHz) spectrometer using chloroform (CDCl3) as a solvent, and chemical shifts were reported as δ values in parts per million with respect to tetramethylsilane (TMS) as an internal reference. FT-IR spectra were recorded using the KBr pellet technique for the analyzed samples and a Thermo Nexus 6700 spectrometer coupled to a Thermo Nicolet Continuum microscope equipped with a 15 × Reflachromat Cassegrain objective, at room temperature in air in the wavenumber range 4000-500 cm -1 , with 64 accumulated scans and at a resolution of 4 cm -1 . The nanogel size, polydispersity index (PDI) and -potential were recorded using the Dynamic Light Scattering (DLS) technique and a Zetasizer Nano ZS from Malvern Instruments. Samples were dissolved in distilled water and the solution was equilibrated for 60 s before data analysis, performed at 37°C. Data shown are an average value of three measurements of each studied nanogel. NG dimensions were also studied with Atomic Force Microscopy (AFM).

FT-IR spectra were recorded using a Thermo Nexus 6700 spectrometer coupled to a Thermo Nicolet Continuum microscope equipped with a 15 × Reflachromat Cassegrain objective, at room temperature in air in the 4000-500 cm -1 wavenumber range with 64 accumulated scans and at a resolution of 4 cm -1 , using the KBr pellet technique for all samples.