293t ace2

Manufactured by BEI Resources

The 293T-ACE2 is a cell line that expresses the human Angiotensin-Converting Enzyme 2 (ACE2) receptor, which is known to be the primary receptor for the SARS-CoV-2 virus. This cell line is commonly used in research applications to study virus-host interactions, vaccine development, and antiviral drug screening.

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Lab products found in correlation

Ccl 136, by American Type Culture Collection (2 mentions) Htb 37, by American Type Culture Collection (2 mentions) Htb 55, by American Type Culture Collection (2 mentions) Ccl 171, by American Type Culture Collection (2 mentions) Vero c1008, by American Type Culture Collection (2 mentions) Hcov oc43, by BEI Resources (2 mentions) Hcov nl63, by BEI Resources (1 mentions) Nr 52725, by BEI Resources (1 mentions) Nr 52511, by BEI Resources (1 mentions) Nr 470, by BEI Resources (1 mentions)

3 protocols using 293t ace2

Evaluating Cell Lines for SARS-CoV-2 Research

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Human rhabdomyosarcoma cell line (RD, ATCC® CCL‐136™), African green monkey kidney cell line Vero C1008 (ATCC® CRL‐1586™), human hepatoma cell line Huh‐7 (a kind gift from Dr. Tianyi Wang at the University of Pittsburgh), and HEK293T expressing angiotensin‐converting enzyme 2 (ACE2) (293T‐ACE2, BEI Resources, NR‐52511) cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM); human fibroblast cell line MRC‐5 (ATCC® CCL‐171™), human lung adenocarcinoma cell line Calu‐3 (ATCC® HTB‐55™), and human colorectal adenocarcinoma cell line (Caco‐2, ATCC® HTB‐37™) were maintained in Eagle's minimum essential medium (ATCC® 30‐2003™). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin antibiotics. Cells were kept at a cell culture incubator (humidified, 5% CO₂/95% air, 37°C). The following reagents were obtained through BEI Resources, NIAID, NIH: HCoV, HCoV‐OC43, NR‐52725; HCoVs, HCoV‐NL63, NR‐470. HCoV‐OC43 was propagated in RD cell line; HCoV‐NL63 was initially propagated in 293T‐ACE2 cell line and accommodated in Vero E6 cell line. HCoV‐229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh‐7 or MRC‐5 cell lines.

Hu Y., Jo H., DeGrado W.F, & Wang J. (2022). Brilacidin, a COVID‐19 drug candidate, demonstrates broad‐spectrum antiviral activity against human coronaviruses OC43, 229E, and NL63 through targeting both the virus and the host cell. Journal of Medical Virology, 94(5), 2188-2200.

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Propagation of Human Coronaviruses in Cell Lines

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Human rhabdomyosarcoma
(RD,
ATCC CCL-136), Vero C1008 (ATCC CRL-1586), Huh-7 (University of Pittsburgh),
and HEK293T expressing ACE2 (293T-ACE2, BEI Resources, NR-52511) cell
lines were maintained in Dulbecco’s modified Eagle’s
medium (DMEM). Human fibroblast Cell Line, MRC-5 (ATCC CCL-171) was
maintained in Eagle’s minimum essential medium (EMEM, ATCC
30–2003). Both media were supplemented with 10% fetal bovine
serum (FBS) and 1% penicillin–streptomycin antibiotics. Cells
were kept at cell culture incubator (humidified, 5% CO₂/95% air, 37 °C). The following reagents were obtained through
BEI Resources, NIAID, NIH: human coronavirus, OC43, NR-52725; human
coronavirus, NL63, NR-470. HCoV-OC43 was propagated in RD cells; HCoV-NL63
was initially propagated in 293T-ACE2 cells and accommodated in Vero
E6 cells. HCoV-229E was obtained from Dr. Bart Tarbet (Utah State
University) and amplified in Huh-7 or MRC-5 cells.

Xia Z., Sacco M., Hu Y., Ma C., Meng X., Zhang F., Szeto T., Xiang Y., Chen Y, & Wang J. (2021). Rational Design of Hybrid SARS-CoV-2 Main Protease Inhibitors Guided by the Superimposed Cocrystal Structures with the Peptidomimetic Inhibitors GC-376, Telaprevir, and Boceprevir. ACS Pharmacology & Translational Science, 4(4), 1408-1421.

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Diverse Cell Lines for Coronavirus Propagation

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Human rhabdomyosarcoma cell line (RD, ATCC^® CCL-136™), African green monkey kidney cell line Vero C1008 (ATCC^® CRL-1586™), Human hepatoma cell line Huh-7 (a kind gift from Dr. Tianyi Wang at University of Pittsburgh), and HEK293T expressing ACE2 (293T-ACE2, BEI Resources, NR-52511) cell lines were maintained in Dulbecco’s modified eagle’s medium (DMEM); Human fibroblast cell line MRC-5 (ATCC^® CCL-171™), Human lung adenocarcinoma cell line Calu-3 (ATCC^® HTB-55™), human Colorectal adenocarcinoma cell line (Caco-2, ATCC® HTB-37™) were maintained in eagle’s minimum essential medium (EMEM, ATCC® 30-2003™). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin antibiotics. Cells were kept at cell culture incubator (humidified, 5% CO₂/95% air, 37 °C). The following reagents were obtained through BEI Resources, NIAID, NIH: human coronavirus, HCoV-OC43, NR-52725; human coronavirus, HCoV-NL63, NR-470. HCoV-OC43 was propagated in RD cell line; HCoV-NL63 was initially propagated in 293T-ACE2 cell line and accommodated in Vero E6 cell line. HCoV-229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh-7 or MRC-5 cell lines.

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